You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml
You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony
forming units (CFU) per mL in the culture. You then do a plate count with these growth results
(no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies;
1:10,000 14 colonies; 1:100,000: no growth
The number of CFU per mL in the original culture was:
1 ml
1 ml
1 ml
1 ml
Original
inoculum
Dilutions
1,000
126
9 ml broth
in each tube
126,000
10,000
140,000
1:10
1 ml
1:100
1 ml
1:1000
1 ml
1 ml
1:10,000
1 ml
1:100,000
1 ml
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