You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml

You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C Total time of exposure (minutes): Number of Microbial Cells Present: 0 100 1 80 3 50 4 42 6.5 26 13 10 21 0
How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.
SPREAD PLATE See the follow up procedure for determining colony counts and express the number of colony forming units (CFU) in scientific notation. Record "TNTC" for plates with colonies that are too numerous to count. CFU/mL-Use scientific notation DILUTION 1:107 1:108 COLONY COUNT ...دما
Fill in the rest of the data to the table attached using the bacteria growth image attached.
A bacterial culture that has been pretreated with penicillin to inhibit its peptidoglycan synthesis is split and put into solutions of various concentrations. The same is done with a control culture with no prior penicillin exposure. Which condition is likely to demonstrate the biggest difference in outcomes between the control culture and the experimental culture? Placing the cultures into an isotonic solution Placing the cultures into a hypotonic solution Placing the cultures into a hypertonic solution The control and experimental cultures should show you the same results in every condition
Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.
There are 10 mL of a culture of a bacteria at 104 cells/mL into 990 mL of the same broth at the same temperature (37° C) It is 8 p.m. the night before your lab which starts at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before your lab? Set up the equation correctly, solve for t, and tell me what time the culture will reach Stationary Phase. t /g = (Log Nt – Log N0) /0.301
Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them. You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose. Are the cells in cultures 1 and 2 deriving all their energy in the same way? (Yes or No) Explain why or why not. Be sure to name the specific processes by which each culture is deriving its energy.
Use the following data to calculate the original bacterial sample. 1 ml Sample of E. coli 110 OF-18 152,000,000 240,000,000 15,200,000 24,000,000 9 ml 1 ml 1/200 OF-100 9 ml DF=Dilution Factor 1/1000 10 1/13100 11.06.1 OF-1000 OF-13501 OF-100100 OF-1.000.000 1 ml 1m mil Over 400 1 ml 152 1ml 1/11/00 DE-10,330 C-TEX 24 1/100/00 1/1/00040 DG-100.000