transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample. a. The dilution factor is 1000. The patient sample contains
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
1 ml of a patient sample was added to 999 ml of media. From this 0.1 ml was transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample.
| a. |
The dilution factor is 1000. The patient sample contains 600 cells/ml |
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| b. |
The dilution factor is 1000. The patient sample contains 600,000 cells/ml |
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| c. |
The dilution factor is 100. The patient sample contains 600,000 cells/ml |
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| d. |
The dilution factor is 10. The patient sample contains 6000 cells/ml |
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| e. |
It cant be determined its too many cells |
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