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How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.
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- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.From a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?
- Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?Fill in the (3) blanks with the correct dilution for each tube in the series.
- How can you make a final dilution of 10-10 using 5 dilution tubes? Outline your steps.How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?
- How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possibleIf you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?