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If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not, what would it be?

If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not, what would it be?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not, what would it be?

**Serial Dilution for Viable Plate Count** The diagram illustrates the process of serial dilution used for viable plate count, specifically focusing on *E. coli*. 1. **Initial Setup:** - The process begins with a sample containing *E. coli*. 2. **Dilution Series:** - A series of tubes are set up, each containing 900 µL of sterile saline. - Starting from the first tube, 100 µL of the *E. coli* sample is added to the first tube, which is labeled as 10⁻¹. This constitutes the first dilution. - The process is repeated sequentially from one tube to the next, transferring 100 µL of solution from the previous tube into the next one. - The tubes are labeled in decreasing concentration from 10⁻¹ to 10⁻⁸. 3. **Plating:** - From selected dilutions (10⁻⁶, 10⁻⁷, and 10⁻⁸), 100 µL is plated onto agar plates. - The plates are expected to grow colonies that reflect the countable number of bacteria present at each dilution level. This method allows for the determination of viable bacteria concentration through colony counting on agar plates. *Figure 1: Serial Dilution for Viable Plate Count*
Transcribed Image Text:**Serial Dilution for Viable Plate Count** The diagram illustrates the process of serial dilution used for viable plate count, specifically focusing on *E. coli*. 1. **Initial Setup:** - The process begins with a sample containing *E. coli*. 2. **Dilution Series:** - A series of tubes are set up, each containing 900 µL of sterile saline. - Starting from the first tube, 100 µL of the *E. coli* sample is added to the first tube, which is labeled as 10⁻¹. This constitutes the first dilution. - The process is repeated sequentially from one tube to the next, transferring 100 µL of solution from the previous tube into the next one. - The tubes are labeled in decreasing concentration from 10⁻¹ to 10⁻⁸. 3. **Plating:** - From selected dilutions (10⁻⁶, 10⁻⁷, and 10⁻⁸), 100 µL is plated onto agar plates. - The plates are expected to grow colonies that reflect the countable number of bacteria present at each dilution level. This method allows for the determination of viable bacteria concentration through colony counting on agar plates. *Figure 1: Serial Dilution for Viable Plate Count*
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