A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg)  is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg)  is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate.

  1. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate?
  2. What is the dilution factor used in this series?
  3. Calculate the number of bacteria in the original patient sample.
  4. What would you do and why, if there were too many colonies to count on each plate?
2 ml
2 ml
2 ml
2 ml
2 ml
1 ml
|1 ml
1 ml
1 ml
mL
Growth of colonies on pour plates after incubation
Too many 5od
to count
colonies
自
ju 8
8 ml
8 ml
8 ml
8 ml
Transcribed Image Text:2 ml 2 ml 2 ml 2 ml 2 ml 1 ml |1 ml 1 ml 1 ml mL Growth of colonies on pour plates after incubation Too many 5od to count colonies 自 ju 8 8 ml 8 ml 8 ml 8 ml
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