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Numeration After spread and pour plating, incubate plates inverted at 35 °C for 24-48 h, and manually count colonies on the agar plates. The acceptable and countable zone is 30-300 CFU/plate. CFU = colony- forming unit. If colonies are <30, count and record it, but we will not use it, because colonies may come from contamination. If colonies are 30-300, count and record it; the final counts will be (final colony number)/final dilution factor of plates, for example, 40 colonies on the plate with final dilution factor 10, and then the final counts are 40/10=40,000,000 CFU/ml = 7.60 log CFU/ml. If colonies on more than two plates are 30-300, count and record each and take average. If colonies are >300, record as TNTC (too numerous to count). Work figure of dilution procedure of plating - 10 CFU/ml E. coli solution onto agar plates. AA 0.1 ml 6.1 ml 1.0 ml 9.0ml BPW (0.1%) 0.1 ml 10¹ final dilution factor

Numeration After spread and pour plating, incubate plates inverted at 35 °C for 24-48 h, and manually count colonies on the agar plates. The acceptable and countable zone is 30-300 CFU/plate. CFU = colony- forming unit. If colonies are <30, count and record it, but we will not use it, because colonies may come from contamination. If colonies are 30-300, count and record it; the final counts will be (final colony number)/final dilution factor of plates, for example, 40 colonies on the plate with final dilution factor 10, and then the final counts are 40/10=40,000,000 CFU/ml = 7.60 log CFU/ml. If colonies on more than two plates are 30-300, count and record each and take average. If colonies are >300, record as TNTC (too numerous to count). Work figure of dilution procedure of plating - 10 CFU/ml E. coli solution onto agar plates. AA 0.1 ml 6.1 ml 1.0 ml 9.0ml BPW (0.1%) 0.1 ml 10¹ final dilution factor

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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3 Enumeration of Bacteria in Broth Suspension by Spread and Pour Plating Numeration After spread and pour plating, incubate plates inverted at 35 °C for 24-48 h, and manually count colonies on the agar plates. The acceptable and countable zone is 30-300 CFU/plate. CFU = colony- forming unit. If colonies are <30, count and record it, but we will not use it, because colonies may come from contamination. If colonies are 30-300, count and record it; the final counts will be (final colony number)/final dilution factor of plates, for example, 40 colonies on the plate with final dilution factor 10-6, and then the final counts are 40/10=40,000,000 CFU/ml = 7.60 log CFU/ml. If colonies on more than two plates are 30-300, count and record each and take average. If colonies are >300, record as TNTC (too numerous to count). Work figure of dilution procedure of plating - 10 CFU/ml E. coli solution onto agar plates. AA 0.1 ml 01 ml E.coli broth Method Spread plating Pour plating 9.9ml BPW (0.1%) 10- 9.9ml BPW (0.1%) 0.1 ml 10³ final dilution factor 10 ml 0.1 ml 10- 9.0ml BPW (0.1%) 9.9 ml BPW (0.1%) 0.1 ml 0.1 ml 10- 10¹ final dilution factor 17 10' final dilution factor Reported count Review Questions 1. A pure bacterial culture was diluted by adding a 0.1 mL aliquot to 9.9 mL water. Then, 0.1 mL of this dilution was plated out by spread plating, yielding 50 colonies. Calculate the CFU per mL in the original culture. 2. A pure bacterial culture was diluted by adding a 1.0 mL aliquot to 9.0 mL water. Then, mL of this dilution was plated out by pour plating, yielding 82 colonies. Calculate the CFU/mL in the original culture.
Transcribed Image Text:3 Enumeration of Bacteria in Broth Suspension by Spread and Pour Plating Numeration After spread and pour plating, incubate plates inverted at 35 °C for 24-48 h, and manually count colonies on the agar plates. The acceptable and countable zone is 30-300 CFU/plate. CFU = colony- forming unit. If colonies are <30, count and record it, but we will not use it, because colonies may come from contamination. If colonies are 30-300, count and record it; the final counts will be (final colony number)/final dilution factor of plates, for example, 40 colonies on the plate with final dilution factor 10-6, and then the final counts are 40/10=40,000,000 CFU/ml = 7.60 log CFU/ml. If colonies on more than two plates are 30-300, count and record each and take average. If colonies are >300, record as TNTC (too numerous to count). Work figure of dilution procedure of plating - 10 CFU/ml E. coli solution onto agar plates. AA 0.1 ml 01 ml E.coli broth Method Spread plating Pour plating 9.9ml BPW (0.1%) 10- 9.9ml BPW (0.1%) 0.1 ml 10³ final dilution factor 10 ml 0.1 ml 10- 9.0ml BPW (0.1%) 9.9 ml BPW (0.1%) 0.1 ml 0.1 ml 10- 10¹ final dilution factor 17 10' final dilution factor Reported count Review Questions 1. A pure bacterial culture was diluted by adding a 0.1 mL aliquot to 9.9 mL water. Then, 0.1 mL of this dilution was plated out by spread plating, yielding 50 colonies. Calculate the CFU per mL in the original culture. 2. A pure bacterial culture was diluted by adding a 1.0 mL aliquot to 9.0 mL water. Then, mL of this dilution was plated out by pour plating, yielding 82 colonies. Calculate the CFU/mL in the original culture.
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