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Can your draw it out and explain it to me please

Can your draw it out and explain it to me please

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Can your draw it out and explain it to me please
36 LAB WEEK 7 Serial Dilutions, and Plate Counting Exercise #2 Pour Plate Method To perform the pour plate method, you will use the dilution tubes from the previous schematic and perform the top three agar plates. You will obtain three empty Petri dishes and label them with the dilution factors. Next, you will pipette 1 ml from each of the 9 ml dilution blanks into their respective plate. Lastly, you will obtain three molten Tryptic Soy Agar (TSA) agar tubes from the hot water bath and pour one agar tube into each plate. Swirl the agar to mix in the water and make the agar disperse evenly in the bottom of the plate. After the agar hardens, give the plates to the instructor for incuba- tion. Next week you will notice that some of the colonies will be on top and grow larger while ones trapped down in the agar will be small and wedge shaped since they do not have as much freedom for growth. DAY 2: RESULTS s E BAS they oddelt sich DE S u bor In the above three circles draw what your pour plates look like after incubation and record the count for each plate scut situs In the above three circles draw what your spread plates look like after incubation and record the count for each plate bacterial cfus/ml Record your final calculation for the concentration of bacteria in the original sample tube from last week Source: Roger L
Transcribed Image Text:36 LAB WEEK 7 Serial Dilutions, and Plate Counting Exercise #2 Pour Plate Method To perform the pour plate method, you will use the dilution tubes from the previous schematic and perform the top three agar plates. You will obtain three empty Petri dishes and label them with the dilution factors. Next, you will pipette 1 ml from each of the 9 ml dilution blanks into their respective plate. Lastly, you will obtain three molten Tryptic Soy Agar (TSA) agar tubes from the hot water bath and pour one agar tube into each plate. Swirl the agar to mix in the water and make the agar disperse evenly in the bottom of the plate. After the agar hardens, give the plates to the instructor for incuba- tion. Next week you will notice that some of the colonies will be on top and grow larger while ones trapped down in the agar will be small and wedge shaped since they do not have as much freedom for growth. DAY 2: RESULTS s E BAS they oddelt sich DE S u bor In the above three circles draw what your pour plates look like after incubation and record the count for each plate scut situs In the above three circles draw what your spread plates look like after incubation and record the count for each plate bacterial cfus/ml Record your final calculation for the concentration of bacteria in the original sample tube from last week Source: Roger L
original sample. A second method that is sometimes used is called a pour plate. With this technique, you may pipette a full ml into a molten agar and then pour this agar into an empty Petri dish. You I can swirl the plate to mix the contents. The molten agar is kept just hot enough so that it does not solidify but not hot enough to kill the bacteria. Once the agar is poured, it will harden quickly and the bacterial cells will be dispersed and trapped into the agar and then grow into colonies during incubation. 1 ml Exercise #1 Spread Plate Method The diagram at the bottom of this page shows a schematic of the dilution series we will perform today using a bacterial culture tube as the original sample. The instructor will walk through the sche- matic on the board to help you calculate out what the dilution factors will be for each bottle, tube and plate. The instructor will also perform a demonstration to show you how to use the instruments consisting of micropipetters and a vortex machine. Next week you will count the colonies and back calculate to see what the bacterial concentration was in the original tube. In order to perform the back calculation, you take the number of colonies and multiply it by the dilution factor on the plate. You also want to drop the negative sign off of the exponent since you are going back the opposite direction. First, you need to determine which plate is the countable plate. A countable plate will have between 30 and 300 colonies on it. Any plate with over 300 colonies will be too crowded or smeared to get a good count and we declare it Too Numerous To Count (TNTC). If a plate has less than 30 colonies on it, then it is discarded for being statistically insignificant. For example, if I have a plate that has 237 colonies on it and the dilution factor is 105, then the calculation would be as follows. The 237 colonies is in the range of 30 to 300 so it is countable. Next, I multiply it by the dilution factor dropping the negative sign which gives me 237 X 105. This is not correct sci- entific notation so I would report 2.37 X107 cfus/ml as my final concentration. If you struggle with scientific notation, take the 237 colonies and put five zeros after it giving you 23700000. Next, move the decimal place between the 2 and 3 and then put in the proper exponent of 107. This would tell me that the original culture tube had a bacterial concentration of approximately 23,700,000 cells/ml. If you happen to have more than one countable plate, then you would average them together. 10² Sample 99 ml 10⁰ = 1 blank Pour Spread 1 ml 10-4 99 ml blank 1 ml 10-5 10-6 1 ml 10-5 9 ml blank 0.1 ml 1 ml 10-6 10 LAB WEEK 7 Serial Dilutions, and Plate Counting Carial Dilution and Standard Plate Count 110-6 9 ml blank 1 ml 1 ml 0.1 ml 107 10° 9 ml blank 1 ml 10-8 0.1 ml 35 Source: Roger Lightner
Transcribed Image Text:original sample. A second method that is sometimes used is called a pour plate. With this technique, you may pipette a full ml into a molten agar and then pour this agar into an empty Petri dish. You I can swirl the plate to mix the contents. The molten agar is kept just hot enough so that it does not solidify but not hot enough to kill the bacteria. Once the agar is poured, it will harden quickly and the bacterial cells will be dispersed and trapped into the agar and then grow into colonies during incubation. 1 ml Exercise #1 Spread Plate Method The diagram at the bottom of this page shows a schematic of the dilution series we will perform today using a bacterial culture tube as the original sample. The instructor will walk through the sche- matic on the board to help you calculate out what the dilution factors will be for each bottle, tube and plate. The instructor will also perform a demonstration to show you how to use the instruments consisting of micropipetters and a vortex machine. Next week you will count the colonies and back calculate to see what the bacterial concentration was in the original tube. In order to perform the back calculation, you take the number of colonies and multiply it by the dilution factor on the plate. You also want to drop the negative sign off of the exponent since you are going back the opposite direction. First, you need to determine which plate is the countable plate. A countable plate will have between 30 and 300 colonies on it. Any plate with over 300 colonies will be too crowded or smeared to get a good count and we declare it Too Numerous To Count (TNTC). If a plate has less than 30 colonies on it, then it is discarded for being statistically insignificant. For example, if I have a plate that has 237 colonies on it and the dilution factor is 105, then the calculation would be as follows. The 237 colonies is in the range of 30 to 300 so it is countable. Next, I multiply it by the dilution factor dropping the negative sign which gives me 237 X 105. This is not correct sci- entific notation so I would report 2.37 X107 cfus/ml as my final concentration. If you struggle with scientific notation, take the 237 colonies and put five zeros after it giving you 23700000. Next, move the decimal place between the 2 and 3 and then put in the proper exponent of 107. This would tell me that the original culture tube had a bacterial concentration of approximately 23,700,000 cells/ml. If you happen to have more than one countable plate, then you would average them together. 10² Sample 99 ml 10⁰ = 1 blank Pour Spread 1 ml 10-4 99 ml blank 1 ml 10-5 10-6 1 ml 10-5 9 ml blank 0.1 ml 1 ml 10-6 10 LAB WEEK 7 Serial Dilutions, and Plate Counting Carial Dilution and Standard Plate Count 110-6 9 ml blank 1 ml 1 ml 0.1 ml 107 10° 9 ml blank 1 ml 10-8 0.1 ml 35 Source: Roger Lightner
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