How would you grow a streptomycin-resistant strain E. coli bacteria was spread on both agar plates and incubated for 24 hours. Pos. Srertomycih Neg. Srettomycir If you wanted to grow up a large quantity of streptomycin resistant E. coli, what would you do next?

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### How Would You Grow a Streptomycin-Resistant Strain

**Step 1: Preparation and Incubation**
- *E. coli* bacteria was spread on both agar plates and incubated for 24 hours.

**Step 2: Observation of Results**
- Two agar plates are shown. One is labeled "Streptomycin Neg",' which means it has no streptomycin, and the other is labeled "Streptomycin Pos",' which means it contains streptomycin.
- The agar plate labeled "Streptomycin Neg" has a uniform bacterial growth
- The agar plate labeled "Streptomycin Pos" has distinct colonies of bacterial growth.

**Step 3: Decision Making**
- If you wanted to grow up a large quantity of streptomycin-resistant *E. coli*, what would you do next?

**Step 4: Multiple-Choice Question**
- Pick a colony of bacteria from the streptomycin-positive plate and continue to grow it on media containing streptomycin.

In conclusion, to cultivate a large quantity of streptomycin-resistant *E. coli*, isolate colonies from the streptomycin-positive agar plate and propagate them on a medium with streptomycin. This selection process ensures that only the resistant strains will thrive.
Transcribed Image Text:### How Would You Grow a Streptomycin-Resistant Strain **Step 1: Preparation and Incubation** - *E. coli* bacteria was spread on both agar plates and incubated for 24 hours. **Step 2: Observation of Results** - Two agar plates are shown. One is labeled "Streptomycin Neg",' which means it has no streptomycin, and the other is labeled "Streptomycin Pos",' which means it contains streptomycin. - The agar plate labeled "Streptomycin Neg" has a uniform bacterial growth - The agar plate labeled "Streptomycin Pos" has distinct colonies of bacterial growth. **Step 3: Decision Making** - If you wanted to grow up a large quantity of streptomycin-resistant *E. coli*, what would you do next? **Step 4: Multiple-Choice Question** - Pick a colony of bacteria from the streptomycin-positive plate and continue to grow it on media containing streptomycin. In conclusion, to cultivate a large quantity of streptomycin-resistant *E. coli*, isolate colonies from the streptomycin-positive agar plate and propagate them on a medium with streptomycin. This selection process ensures that only the resistant strains will thrive.
**Multiple Choice Question**

Please select the appropriate action from the options below pertaining to bacterial colony growth on streptomycin plates:

- **Option 1:** Pick a colony of bacteria from the streptomycin positive plate and grow it on a streptomycin negative plate.
- **Option 2:** Pick a colony of bacteria from the streptomycin negative plate and grow it on a streptomycin negative plate.
- **Option 3:** Pick a colony of bacteria from the streptomycin positive plate and grow it on a streptomycin positive plate.
- **Option 4:** Pick a colony of bacteria from the streptomycin negative plate and grow it on a streptomycin positive plate.

This multiple-choice question is part of a quiz and requires knowledge of bacterial growth on selective media containing the antibiotic streptomycin.

**Instructions:**
- Carefully read each option.
- Select the action that correctly describes the experimental setup.

---

In this image, there are no graphs or diagrams to explain. The provided information consists solely of text-based multiple-choice questions related to a microbiology experiment.
Transcribed Image Text:**Multiple Choice Question** Please select the appropriate action from the options below pertaining to bacterial colony growth on streptomycin plates: - **Option 1:** Pick a colony of bacteria from the streptomycin positive plate and grow it on a streptomycin negative plate. - **Option 2:** Pick a colony of bacteria from the streptomycin negative plate and grow it on a streptomycin negative plate. - **Option 3:** Pick a colony of bacteria from the streptomycin positive plate and grow it on a streptomycin positive plate. - **Option 4:** Pick a colony of bacteria from the streptomycin negative plate and grow it on a streptomycin positive plate. This multiple-choice question is part of a quiz and requires knowledge of bacterial growth on selective media containing the antibiotic streptomycin. **Instructions:** - Carefully read each option. - Select the action that correctly describes the experimental setup. --- In this image, there are no graphs or diagrams to explain. The provided information consists solely of text-based multiple-choice questions related to a microbiology experiment.
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