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Marie diluted her phage lysate from 10° to 109. She completed the titer assay protocol using these dilutions. The results showed that none of the plates contained bacteria. Assume that Marie did her dilutions correctly. What is the most likely explanation for this result? O her phage had become inactivated by being stored in the refrigerator too long Oshe added the top agar to the bacteria when the top agar was much too hot O the phage buffer that she used was contaminated with a small amount of a different phage O she did not mix the phage and bacteria well before plating them O the top agar that she used was contaminated with a type of bacteria different than M. smeg

Marie diluted her phage lysate from 10° to 109. She completed the titer assay protocol using these dilutions. The results showed that none of the plates contained bacteria. Assume that Marie did her dilutions correctly. What is the most likely explanation for this result? O her phage had become inactivated by being stored in the refrigerator too long Oshe added the top agar to the bacteria when the top agar was much too hot O the phage buffer that she used was contaminated with a small amount of a different phage O she did not mix the phage and bacteria well before plating them O the top agar that she used was contaminated with a type of bacteria different than M. smeg

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Marie diluted her phage lysate from 100 to 10-⁹. She completed the titer assay protocol using these dilutions. The results showed that none of the plates contained bacteria. Assume that Marie did her dilutions correctly. What is the most likely explanation for this result? O her phage had become inactivated by being stored in the refrigerator too long O she added the top agar to the bacteria when the top agar was much too hot O the phage buffer that she used was contaminated with a small amount of a different phage O she did not mix the phage and bacteria well before plating them O the top agar that she used was contaminated with a type of bacteria different than M. smeg
Transcribed Image Text:Marie diluted her phage lysate from 100 to 10-⁹. She completed the titer assay protocol using these dilutions. The results showed that none of the plates contained bacteria. Assume that Marie did her dilutions correctly. What is the most likely explanation for this result? O her phage had become inactivated by being stored in the refrigerator too long O she added the top agar to the bacteria when the top agar was much too hot O the phage buffer that she used was contaminated with a small amount of a different phage O she did not mix the phage and bacteria well before plating them O the top agar that she used was contaminated with a type of bacteria different than M. smeg
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