Katelyn had been working for Dr. Johnson for a month, and while she had become quite good at measuring inhibition zones, she didn’t know why she was doing all this work. She had gotten very curious after she began doing all the measurements on a new set of antibiotics. # is experiment involved infecting mice with MRSA and tracking how the MRSA grew over time. Data were collected by counting the cells of MRSA taken from $ uid samples from the mice. # e cells were measured by taking one gram of the $ uid and spreading it over plates, but now Katelyn counted the colonies that grew on the plate after 24 hours. Because there were so many, she actually measured the colonies as “log CFU/g.” A CFU is a colony forming unit, or essentially a cell that will divide into a colony that can be seen. Because there can be so many, Katelyn measured them on a logarithmic (log) scale. # e raw data in her lab notebook looked like the following: Table 1. E% ect of treatment on MRSA in mice after 24 hours of drug treatment as log CFU/g. Trial Control Treatment FtsZ inhibitor Imipenem FtsZ inhibitor + imipenem 1 9.11 7.55 6.98 2.21 2 8.25 8.12 8.12 4.55 3 9.05 9.27 9.01 7.98 4 9.37 8.02 8.33 5.64 5 8.80 7.65 7.64 1.25 6 9.25 8.3 7.77 9.98 7 9.41 7.99 8.21 6.78 8 9.11 7.71 7.98 3.45 9 8.61 8.22 7.68 2.45 10 9.12 8.11 8.21 1.01 Questions 1. What do you think the experimental question is? 2. What hypotheses can you come up with to answer the experimental question? 3. What predictions would you make for each hypothesis? 4. Looking at the data in Table 1, what do these numbers mean? (Keep in mind a log value means each integer increase is actually a ten-fold increase in the number of cells.) 5. What do you think FtsZ inhibitor and imipenem are?
Katelyn had been working for Dr. Johnson for a month, and while she had become quite good at measuring inhibition
zones, she didn’t know why she was doing all this work. She had gotten very curious after she began doing all the
measurements on a new set of antibiotics. # is experiment involved infecting mice with MRSA and tracking how the
MRSA grew over time.
Data were collected by counting the cells of MRSA taken from $ uid samples from the mice. # e cells were measured
by taking one gram of the $ uid and spreading it over plates, but now Katelyn counted the colonies that grew on the
plate after 24 hours. Because there were so many, she actually measured the colonies as “log CFU/g.” A CFU is a
colony forming unit, or essentially a cell that will divide into a colony that can be seen. Because there can be so many,
Katelyn measured them on a logarithmic (log) scale. # e raw data in her lab notebook looked like the following:
Table 1. E% ect of treatment on MRSA in mice after 24 hours of drug treatment as log CFU/g.
Trial Control
Treatment
FtsZ inhibitor Imipenem
FtsZ inhibitor +
imipenem
1 9.11 7.55 6.98 2.21
2 8.25 8.12 8.12 4.55
3 9.05 9.27 9.01 7.98
4 9.37 8.02 8.33 5.64
5 8.80 7.65 7.64 1.25
6 9.25 8.3 7.77 9.98
7 9.41 7.99 8.21 6.78
8 9.11 7.71 7.98 3.45
9 8.61 8.22 7.68 2.45
10 9.12 8.11 8.21 1.01
Questions
1. What do you think the experimental question is?
2. What hypotheses can you come up with to answer the experimental question?
3. What predictions would you make for each hypothesis?
4. Looking at the data in Table 1, what do these numbers mean? (Keep in mind a log value means each integer
increase is actually a ten-fold increase in the number of cells.)
5. What do you think FtsZ inhibitor and imipenem are?
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