You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculatedwith your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made surethat all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage inthe media. What is the most likely explanation?O You used the wrong bacteria or the wrong bacteriophage.There is an issue with the phage batch.O There is a problem with your bacterial culture of B. cereus.O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.

Answered: You are attempting to propagate…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated
with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure
that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in
the media. What is the most likely explanation?
O You used the wrong bacteria or the wrong bacteriophage.
There is an issue with the phage batch.
O There is a problem with your bacterial culture of B. cereus.
O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.

Expert Solution

Step 1

Bacteriophage : these are the viruses that attack on barterial cells and use them to produce more copies of themselves.

When we inoculate the Bacillus cereus with their bacteriophages, the bacteriophages attack on the bacterial cells and start multiplying in them.

The viral cells transfers their genetic material into the bacterial cells, this viral DNA incorporates with the DNA of bacterial cell and takes over the control of bacterial cell.

Now this viral DNA directs the bacterial cell to produce viral proteins and genetic material, these proteins and viral DNA combine and came out of the bacterial cell, as new viruses.

In this way viral particles multiply in the bacterial cells.

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