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- Why is it so important to develop tests to ID bacteriaYou are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in the media. What is the most likely explanation? O You used the wrong bacteria or the wrong bacteriophage. There is an issue with the phage batch. O There is a problem with your bacterial culture of B. cereus. O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.You are developing a hypothetical culture medium for the isolation of salmonella Typhimurium. How will you use these unique characteristics to produce a culture medium that is both selective and differential for the isolation of presumptive Salmonella Typhimurium?
- During the process of conjugation between two bacteria, each bacterium transfers some of its DNA into the other bacterium. True FalseWhy is it important to follow sterile technique? What can happen to cause gram-positive bacteria to appear gram-negative?When T4 phage isolated from E. coli strain B infect E. coli strain K, what will be the EOP (efficient of plating)? Explain why. Fe V
- What is the importance of the agar overlay technique in virus isolation?Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plateYou’ve collected a soil sample from an unexplored island and cultured a new bacterial species by growing it on complete media. Being a wild-type strain, you assume it is prototrophic. What would you do to determine whether this is true?