In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?

Q: Is it important to have fewer phages than bacterial host cells when doing a quantitative plaque…

A: A virus is an infective agent, which is sub-microscopic in nature. It has the capability of…

Q: In Griffith's experiment, the S type bacteria are harmless, but can be transformed into the…

A: In Griffith's experiment, there was a mix of  heat-killed S-strain bacteria with live, harmless…

Q: You are studying a microorganism that contains a chloramphenicol acetyltransferase (CAT) enzyme, and…

A: As parent have CAT enzyme means it grows on chloramphenicol containing plate. And we want clone with…

Q: Which of the following enzyme is used in PCR?a) Taq DNA polymeraseb) HRPc) EcoRId) EcoRII

A: Polymerase chain reaction (PCR) is a laboratory technique, which is used to make multiple copies of…

Q: Why is it important to label plates on the bottom rather than the lid?

A: Introduction :-  For studying the various different types of bacterial cell , first we have to…

Q: In terms of microbial pathogenicity, molecular Koch's postulates are a set of experimental criteria…

A: Koch's postulates are four criteria that were designed to establish the causal relationship between…

Q: Using phages that came from E. coli with the genotype al+ be+, you infect a strain ofE. coli that is…

A: Auxotrophs are the term used for the organism or bacteria that cannot express a gene due to mutation…

Q: Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only…

A: PCR polymerase chain reaction is a biotechnology technique used for amplification of DNA.

Q: You are counting plaques on your plaque assay plates made from serial dilutions of your high titer…

A: A serial dilution is any dilution in which the concentration decreases by the same dilutions in each…

Q: Griffith's Experiments using Streptococcus pneumonia rough and smooth strains concluded which of the…

A: Streptococcus pneumoniae also known as pneumococcus, is a Gram-positive spherical-shaped bacterium…

Q: ou followed all the steps correctly with a kit you bought last week. However, NO purified DNA came…

A: Plasmids exist naturally in bacterial cells . In some cases bacteria need to pick up plasmid from…

Q: All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1…

A: The Luria-Delbruck Experiment ---This experiment performed in 1943  demonstrate that in bacteria…

Q: What is the nucleic extraction technique for the identification of Candida albicans?

A: Isolation of genomic DNA is a tedious and sensitive process, which requires proper procedure and…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

Q: In a plaque assay, a phage has undergone _________ cycle when plaques are observed on the agar lawn…

A: Viruses are obligate intracellular parasites that requires a living host cell to grow and multiply.…

Q: The cath sample was both filtered to remove all bacteria, and then treated with DNase.  Based on…

A: A cath or catheter is a thin hollow tube, which is a medical equipment that is used for various…

Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…

A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…

Q: You are trying to isolate a mutant of wild-type E. coli thatrequires histidine for growth. This can…

A: E.coli. is a type of bacteria. Bacteria are unicellular and prokaryotic organisms that live in a…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: You are running your first ELISA and make some mistakes. What would happen if each of the following…

A: The objective of the question is to understand the impact of various mistakes that can occur during…

Q: you transform bacteria with the pGLO plasmid. What do you need to add to the media to get them to…

A: Green Fluorescent Protein (GFP) is obtained from jelly fish (Aequorea victoria). It has excitation…

Q: How do you know if you Azotobacter bacteria isolation was successful? Could you please list all…

A: Azotobacter is a genus of free-living, nitrogen-fixing bacteria that are found in soil and water.…

Q: You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of…

A: Staphylococcus aureus is highly prone for antibiotic resistance. Therefore while handling these…

Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…

A: Dilution is the process of making a solution weaker or less concentrated.

Q: LB (Lysogeny broth) medium for the growth of E. coli,

A: Pseudomonas putida is a kind of rod-shaped, Gram-negative, non-fermenting bacterium which is present…

Q: Why would you use one single colony for both streaks on a plate and inoculate a culture for plasmid…

A: Introduction Plasmids are important for the transformation of bacteria. They are used as vectors and…

Q: Which of the following experimental techniques was used by Hershey and Chase to establish the…

A: Answer : option 3) Isotope labelling is correct.

Q: Which of the following is not associated with gene therapy SCID patients A) utilizes an enzyme to…

A: Severe combined immunodeficiency (SCID): it is an inherited primary immunodeficiency disease (PIDD)…

Q: What is the role of the following in the alkaline plasmid screen?    G buffer (Cell Suspension…

A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…

Q: 10) With the disk test, what specifically are you looking for to know if a bacteria is susceptible…

A: The disk test is done to find which bacteria is susceptible to which antibiotic. This test is also…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: 1. Which of the following bacterial species is not naturally competent? A) Bacillus subtilis B)…

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…

Q: Does the addition of E. coli change the dilution in the calculation of PFU/mL in the original phage…

A: Plaque refers to the clear or visible zone formed by the viral sample when it is introduced to the…

Q: What are Bacterial Plasmid Vectors ?

A:

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.