You are running your first ELISA and make some mistakes. What would happen if each of the following happened in isolation? Would the mistake inherently make the data unusable? (Tip: You might find the trouble shooting table from an ELISA protocol helpful, one is posted to ICON) a) You only add 50ul instead of 100 ul of both standards and experimental samples b) You use the same pipette tips to add the HRP-detection antibody and TMB substrate. c) You forget about your plate after adding your sample to a pre-coated plate and it incubates for an extra 4 hours. d) You forget about your plate after adding the TMB substrate and it incubates for an extra 4 hours. e) After adding the HRP-Detection antibody, you are so excited to see the result, you decide to wash just once and then add the TMB reagent.

You are running your first ELISA and make some mistakes. What would happen if each of the following happened in isolation? Would the mistake inherently make the data unusable? (Tip: You might find the trouble shooting table from an ELISA protocol helpful, one is posted to ICON) a) You only add 50ul instead of 100 ul of both standards and experimental samples b) You use the same pipette tips to add the HRP-detection antibody and TMB substrate. c) You forget about your plate after adding your sample to a pre-coated plate and it incubates for an extra 4 hours. d) You forget about your plate after adding the TMB substrate and it incubates for an extra 4 hours. e) After adding the HRP-Detection antibody, you are so excited to see the result, you decide to wash just once and then add the TMB reagent.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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