a)similarity between Western blot and ELISA is a.)both have two 1° Ab to protein of interest b.)both use SDS-PAGE c.)both use centrifugation d.)both detect protein of interest by measure of product made by enzyme B.)the second antibody added in ELISA has this property a.)it is bound to a large, heavy molecule b.)it is added after SDS-PAGE c.Jit recognizes primary antibody d.)it recognizes protein of interest
a)similarity between Western blot and ELISA is a.)both have two 1° Ab to protein of interest b.)both use SDS-PAGE c.)both use centrifugation d.)both detect protein of interest by measure of product made by enzyme B.)the second antibody added in ELISA has this property a.)it is bound to a large, heavy molecule b.)it is added after SDS-PAGE c.Jit recognizes primary antibody d.)it recognizes protein of interest
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:**Biochemistry Classwork**
---
**Problem 1**
**a) Similarity between Western blot and ELISA is:**
- a.) both have two 1° Ab to protein of interest
- b.) both use SDS-PAGE
- c.) both use centrifugation
- d.) both detect protein of interest by measure of product made by enzyme
**b) The second antibody added in ELISA has this property:**
- a.) it is bound to a large, heavy molecule
- b.) it is added after SDS-PAGE
- c.) it recognizes primary antibody
- d.) it recognizes protein of interest
---
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