a)similarity between Western blot and ELISA is a.)both have two 1° Ab to protein of interest b.)both use SDS-PAGE c.)both use centrifugation d.)both detect protein of interest by measure of product made by enzyme B.)the second antibody added in ELISA has this property a.)it is bound to a large, heavy molecule b.)it is added after SDS-PAGE c.Jit recognizes primary antibody d.)it recognizes protein of interest

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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**Biochemistry Classwork**

---

**Problem 1**

**a) Similarity between Western blot and ELISA is:**
  - a.) both have two 1° Ab to protein of interest
  - b.) both use SDS-PAGE
  - c.) both use centrifugation
  - d.) both detect protein of interest by measure of product made by enzyme

**b) The second antibody added in ELISA has this property:**
  - a.) it is bound to a large, heavy molecule
  - b.) it is added after SDS-PAGE
  - c.) it recognizes primary antibody
  - d.) it recognizes protein of interest

---

No graphs or diagrams are present in the provided image.
Transcribed Image Text:**Biochemistry Classwork** --- **Problem 1** **a) Similarity between Western blot and ELISA is:** - a.) both have two 1° Ab to protein of interest - b.) both use SDS-PAGE - c.) both use centrifugation - d.) both detect protein of interest by measure of product made by enzyme **b) The second antibody added in ELISA has this property:** - a.) it is bound to a large, heavy molecule - b.) it is added after SDS-PAGE - c.) it recognizes primary antibody - d.) it recognizes protein of interest --- No graphs or diagrams are present in the provided image.
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