Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS.You next take 0.5 mL of Dr. Riggs's sample from step 1 and add it to 9.5 mL of PBS.You load your hemacytometer with the dilution you made in step 2, look at it under the microscope, but there are still too many cells to count.You now take 0.2mL of sample from step 2 and add it to 5ml of PBS.Using a hemacytometer, you load your sample and count the following number of red blood cells in 1 chamber (5 squares):chamber 1 = 25, 25, 30, 20, 30 If this mouse has 2 mL of blood in it’s body, how many red blood cells does it have?The options are 1.49X10^62.97X10^6not enough information to tell1.49X10^92.97X10^9

Answered: Dr. Riggs took 1ml of whole mouse blood…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS.
You next take 0.5 mL of Dr. Riggs's sample from step 1 and add it to 9.5 mL of PBS.
You load your hemacytometer with the dilution you made in step 2, look at it under the microscope, but there are still too many cells to count.
You now take 0.2mL of sample from step 2 and add it to 5ml of PBS.
Using a hemacytometer, you load your sample and count the following number of red blood cells in 1 chamber (5 squares):

chamber 1 = 25, 25, 30, 20, 30

If this mouse has 2 mL of blood in it’s body, how many red blood cells does it have?

The options are

1.49X10^6

2.97X10^6

not enough information to tell

1.49X10^9

2.97X10^9

Expert Solution

Step 1: Calculate the Average Number of Cells in the Hemacytometer

The first step is to calculate the average number of cells counted in the hemacytometer. The counts from the 5 squares are 25, 25, 30, 20, and 30. The average can be calculated as follows:

$text Average Count end text equals fraction numerator 25 plus 25 plus 30 plus 20 plus 30 over denominator 5 end fraction equals 26$

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