In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC, PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in the figure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal from Filter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1 Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2 You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose 4 and justify your selection. # Filter type Wavelength A Long pass 650 nm B Short pass 530 nm C Long pass 490 nm D Band pass 550-590 nm E Long pass 560 nm F Short pass 640 nm G Long pass 620 nm H Band pass 620-640 nm I Short pass 590 nm
In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC, PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in the figure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal from Filter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1 Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2 You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose 4 and justify your selection. # Filter type Wavelength A Long pass 650 nm B Short pass 530 nm C Long pass 490 nm D Band pass 550-590 nm E Long pass 560 nm F Short pass 640 nm G Long pass 620 nm H Band pass 620-640 nm I Short pass 590 nm
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,
PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in the
figure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal from
Filter 3 and the PE-Cy 5 signal from Filter 4.
PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in the
figure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal from
Filter 3 and the PE-Cy 5 signal from Filter 4.
Part 4.1
Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal.
Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal.
Part 4.2
You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose
4 and justify your selection.
# Filter type Wavelength
A Long pass 650 nm
B Short pass 530 nm
C Long pass 490 nm
D Band pass 550-590 nm
E Long pass 560 nm
F Short pass 640 nm
G Long pass 620 nm
H Band pass 620-640 nm
I Short pass 590 nm
You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose
4 and justify your selection.
# Filter type Wavelength
A Long pass 650 nm
B Short pass 530 nm
C Long pass 490 nm
D Band pass 550-590 nm
E Long pass 560 nm
F Short pass 640 nm
G Long pass 620 nm
H Band pass 620-640 nm
I Short pass 590 nm
Transcribed Image Text:Emission intensity
FITC
PE
PE-TR
PE-Cy5
500 550 600 650 700 750
Emission wavelength (nm)
Filter 1
Filter 2
Filter 3
Filter 4
ネネネネー
From sample
FITC
PE
PE-TR
PE-CYS
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON