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- Why ammonium chloride an inhibitor for lysosome?What other method could be used to purify lysozyme from white using its unusual pl?in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugation
- Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Type of Centrifugation* Type of fraction *Give 2 advantages of using this type of centrifuge.*Give 2 disadvantages of using this type of centrifuge.Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
- You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you needto centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of yourcentrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At whatrpm should you spin the centrifuge for pelleting the cells?If you need 2.5 mg/liter, 2,4-D in 1 liter of medium and the stock solution of 2,4-D contains 10 mg/100 ml, how much stock solution do you need? why BAP is preferred over other cytokinin during commercial plant tissue culturedescribe how lysozyme may or may not be separated by thin-layer chromatography
- (d) If one bacteria cell divides every 30 minutes, calculate the number of cells after (a) Assuming the bacterial cell divides every 20 minutes under standard conditions, (c) How many hours will it take to produce 8192 cells under the same conditions. If a bacterial culture with initial cells number (No) increases by a factor of 2 in one generation and n is the time as given by the formula in equation 1. N = (2")No -----(1) calculate the number of cell division in 4 hours. (b) Calculate the number of cells produced after 4 lhours. hours.In Figure 5-5,a. Why do A− and B− cells, by themselves, not formcolonies on the plating medium?b. What genetic event do the purple colonies in themiddle plate represent?In the context of flow cytometry data: It is clear that Lactobacillus cells can be detected based on their FSC (forward scatter) and SSC (side scatter) signal since it is above the noise. Thus, they could be quantified using these parameters without staining. Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain heterotrophs?