Is Bacillis brevis a gram-positive or gram-negative bacterial cell? Based on this response, describe the cell wall structure and how it differs from the other type. Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer. What factor(s) did you take into consideration when selecting the number of passes?
-
Bacillis brevis are lysed using a valve-type homogenizer. The extent of disruption depends on the applied pressure and the number of passes through the homogenizer chamber and can be described by the equation, ln(1 − ?) = −????.
-
Is Bacillis brevis a gram-positive or gram-negative bacterial cell? Based on this response,
describe the cell wall structure and how it differs from the other type.
-
Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer.
-
What factor(s) did you take into consideration when selecting the number of passes?
-
Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative?
-
What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a?
-
If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?
-
Answer as many as you can please
Step by step
Solved in 4 steps
