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Using the disc diffusion table in the previous question, which bacterium was most resistant to the hydrophlic antiseptics and disinfectants tested? Explain Gram-positive_ Bacillus_; protection provided by thick layer of peptidoglycan O Gram-positive_ Bacillus_; protection provided by lipid rich outer membrane of cell wall O Gram-negative_ E. coli_; protection provided by thick layer of peptidoglycan Gram-negative_ E.coli_; protection provided by lipid rich outer membrane of cell wall

Using the disc diffusion table in the previous question, which bacterium was most resistant to the hydrophlic antiseptics and disinfectants tested? Explain Gram-positive_ Bacillus_; protection provided by thick layer of peptidoglycan O Gram-positive_ Bacillus_; protection provided by lipid rich outer membrane of cell wall O Gram-negative_ E. coli_; protection provided by thick layer of peptidoglycan Gram-negative_ E.coli_; protection provided by lipid rich outer membrane of cell wall

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
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Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: | | 1:10 diluted bleach (BL) | Lab Disinfectant (DIS) | Isopropanol (ISP) | Lab soap (SO) | Lysol (LYS) | Tincture of iodine (T of I) | |--------------------|--------------------------|-------------------------|-------------------|---------------|-------------|-----------------------------| | Diameter in mm of zone of inhibition | | *E. coli* | 40 mm | 20 mm | 10 mm | 5 mm | 15 mm | 60 mm | | *Bacillus cereus* | 50 mm | 35 mm | 15 mm | 8 mm | 20 mm | 70 mm | ### Explanation of the Table: - **Table Structure**: The table shows the effectiveness of different antiseptics and disinfectants, measured by the diameter in millimeters (mm) of the zone of inhibition they produce against two types of bacteria: *E. coli* and *Bacillus cereus*. - **Columns**: - The first column lists the substances tested: 1:10 diluted bleach, Lab Disinfectant, Isopropanol, Lab soap, Lysol, and Tincture of iodine. - Each subsequent column shows the size of the inhibition zone for each substance and bacteria pair. - **Rows**: - The second and third rows provide measurements for the inhibition zone diameter for *E. coli* and *Bacillus cereus*, respectively, for each substance. ### Key Observations: - Tincture of iodine has the largest zone of inhibition for both *E. coli* (60 mm) and *Bacillus cereus* (70 mm), indicating high effectiveness. - Lab soap has the smallest zones of inhibition, indicating limited effectiveness against both bacteria. - In general, *Bacillus cereus* shows larger zones of inhibition compared to *E. coli* for most substances.
Transcribed Image Text:Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: | | 1:10 diluted bleach (BL) | Lab Disinfectant (DIS) | Isopropanol (ISP) | Lab soap (SO) | Lysol (LYS) | Tincture of iodine (T of I) | |--------------------|--------------------------|-------------------------|-------------------|---------------|-------------|-----------------------------| | Diameter in mm of zone of inhibition | | *E. coli* | 40 mm | 20 mm | 10 mm | 5 mm | 15 mm | 60 mm | | *Bacillus cereus* | 50 mm | 35 mm | 15 mm | 8 mm | 20 mm | 70 mm | ### Explanation of the Table: - **Table Structure**: The table shows the effectiveness of different antiseptics and disinfectants, measured by the diameter in millimeters (mm) of the zone of inhibition they produce against two types of bacteria: *E. coli* and *Bacillus cereus*. - **Columns**: - The first column lists the substances tested: 1:10 diluted bleach, Lab Disinfectant, Isopropanol, Lab soap, Lysol, and Tincture of iodine. - Each subsequent column shows the size of the inhibition zone for each substance and bacteria pair. - **Rows**: - The second and third rows provide measurements for the inhibition zone diameter for *E. coli* and *Bacillus cereus*, respectively, for each substance. ### Key Observations: - Tincture of iodine has the largest zone of inhibition for both *E. coli* (60 mm) and *Bacillus cereus* (70 mm), indicating high effectiveness. - Lab soap has the smallest zones of inhibition, indicating limited effectiveness against both bacteria. - In general, *Bacillus cereus* shows larger zones of inhibition compared to *E. coli* for most substances.
### Understanding Bacterial Resistance **Question:** Using the disc diffusion table in the previous question, which bacterium was most resistant to the hydrophilic antiseptics and disinfectants tested? Explain. 1. **Gram-positive Bacillus**: Protection provided by a thick layer of peptidoglycan. 2. **Gram-positive Bacillus**: Protection provided by a lipid-rich outer membrane of the cell wall. 3. **Gram-negative E. coli**: Protection provided by a thick layer of peptidoglycan. 4. **Gram-negative E. coli**: Protection provided by a lipid-rich outer membrane of the cell wall. --- This question examines the resistance mechanisms of different types of bacteria when exposed to hydrophilic antiseptics and disinfectants. The explanations focus on the structural components relevant to each type of bacterium: - **Gram-positive bacteria** typically have a thick peptidoglycan layer which provides protection. - **Gram-negative bacteria** have a lipid-rich outer membrane that serves as a barrier against certain chemicals. Understanding which structural feature provides resistance is crucial for determining effective antimicrobial strategies.
Transcribed Image Text:### Understanding Bacterial Resistance **Question:** Using the disc diffusion table in the previous question, which bacterium was most resistant to the hydrophilic antiseptics and disinfectants tested? Explain. 1. **Gram-positive Bacillus**: Protection provided by a thick layer of peptidoglycan. 2. **Gram-positive Bacillus**: Protection provided by a lipid-rich outer membrane of the cell wall. 3. **Gram-negative E. coli**: Protection provided by a thick layer of peptidoglycan. 4. **Gram-negative E. coli**: Protection provided by a lipid-rich outer membrane of the cell wall. --- This question examines the resistance mechanisms of different types of bacteria when exposed to hydrophilic antiseptics and disinfectants. The explanations focus on the structural components relevant to each type of bacterium: - **Gram-positive bacteria** typically have a thick peptidoglycan layer which provides protection. - **Gram-negative bacteria** have a lipid-rich outer membrane that serves as a barrier against certain chemicals. Understanding which structural feature provides resistance is crucial for determining effective antimicrobial strategies.
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