Other tests: Hydrogen sulfide production: DNase (DNA depolymerization): positive Arginine (arginine decarboxylation): positive (weakly) Lysine (lysine decarboxylation): Ornithine (ornithine decarboxylation):

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Help with my unknown lab report for staphylococcus aureus.
Other tests:
Hydrogen sulfide production:
DNase (DNA depolymerization): positive
Arginine (arginine decarboxylation): positive (weakly)
Lysine (lysine decarboxylation):
Ornithine (ornithine decarboxylation):
Notes:
Coagulase: positive
Transcribed Image Text:Other tests: Hydrogen sulfide production: DNase (DNA depolymerization): positive Arginine (arginine decarboxylation): positive (weakly) Lysine (lysine decarboxylation): Ornithine (ornithine decarboxylation): Notes: Coagulase: positive
Morphological Characteristics
Gram reaction: positive
Cell shape: coccus
Cell size: 0.5-1.5 um
Acid-fast: negative
Arrangement: singly, pairs, irregular clusters
Endospores present: negative
Capsule present: negative
Motility: negative
Cultural Characteristics
Optimum temperature: 30-370 C (grows 15o to 450 C)
Oxygen requirement: facultative anaerobe
Colony morphology on TSA plate: smooth, raised, circular, >5mm dia,
entire, white, cream, some colonies may show weak yellow to orange
Pattern of growth on TSA slant: effuse
Pattern of growth in TSB: uniformly turbid, ring pellicle
Type of hemolysis on BAP: Beta (after 48 hrs)
Physiological Characteristics
Oxidation/Fermentation tests
Glucose (dextrose) fermentation: Acid
Lactose fermentation: Acid
Mannitol fermentation: Acid
Sucrose fermentation: acid
Oxidation/Fermentation of glucose: O+/F+
Methyl Red (fermentation via mixed acid pathway):
Voges-Proskauer (fermentation via butanediol pathway:
Citrate utilization:
Nitrate reduction: positive
Oxidase test: negative
Hydrolytic and Degradative tests:
Catalase (breakdown of hydrogen peroxide): positive
Gelatin (gelatin hydrolysis):
Starch (amylase hydrolysis):
Skim milk (casein hydrolysis):
Urea (urea hydrolysis): positive (weakly)
Indole (tryptophan hydrolysis):
Phenylalanine (phenylalanine deamination):
Transcribed Image Text:Morphological Characteristics Gram reaction: positive Cell shape: coccus Cell size: 0.5-1.5 um Acid-fast: negative Arrangement: singly, pairs, irregular clusters Endospores present: negative Capsule present: negative Motility: negative Cultural Characteristics Optimum temperature: 30-370 C (grows 15o to 450 C) Oxygen requirement: facultative anaerobe Colony morphology on TSA plate: smooth, raised, circular, >5mm dia, entire, white, cream, some colonies may show weak yellow to orange Pattern of growth on TSA slant: effuse Pattern of growth in TSB: uniformly turbid, ring pellicle Type of hemolysis on BAP: Beta (after 48 hrs) Physiological Characteristics Oxidation/Fermentation tests Glucose (dextrose) fermentation: Acid Lactose fermentation: Acid Mannitol fermentation: Acid Sucrose fermentation: acid Oxidation/Fermentation of glucose: O+/F+ Methyl Red (fermentation via mixed acid pathway): Voges-Proskauer (fermentation via butanediol pathway: Citrate utilization: Nitrate reduction: positive Oxidase test: negative Hydrolytic and Degradative tests: Catalase (breakdown of hydrogen peroxide): positive Gelatin (gelatin hydrolysis): Starch (amylase hydrolysis): Skim milk (casein hydrolysis): Urea (urea hydrolysis): positive (weakly) Indole (tryptophan hydrolysis): Phenylalanine (phenylalanine deamination):
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