Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer.What factor(s) did you take into consideration when selecting the number of passes?Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative?What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a?If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend? 1. Bacillis brevis are lysed using a valve-type homogenizer. The extent of disruptiondepends on the applied pressure and the number of passes through the homogenizerchamber and can be described by the equation, In(1 - R) = -kN pa

Bacillus brevis is a rod-shaped bacteria that is widely found in soil, water, and fermented foods.It…

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer.
What factor(s) did you take into consideration when selecting the number of passes?
Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative?
What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a?
If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?

1. Bacillis brevis are lysed using a valve-type homogenizer. The extent of disruption
depends on the applied pressure and the number of passes through the homogenizer
chamber and can be described by the equation, In(1 - R) = -kN pa

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