Bacterial density (cells/mL) Glucose used up Lactose used up Glucose + Lactose added 0 4 2 4 5 A B Time (h) с -x 10 110 VD

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.

Bacterial density (cells/mL)
Glucose
used up
Lactose
used up
Glucose +
Lactose
added
0 4 2 4
5
A
B
Time (h)
с
-x
10
110
VD
Transcribed Image Text:Bacterial density (cells/mL) Glucose used up Lactose used up Glucose + Lactose added 0 4 2 4 5 A B Time (h) с -x 10 110 VD
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