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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
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- [protein] volume [protein] [protein] in to Sample Lysate in "gel- load 5 in 20 protein in Name volume assay* lysate ready" ul sample protein 3.9 2 µl Hg/ml Tilapia 19.9 10 μι Hg/ml 18.2 2 µl Hg/ml Cod >20 14 10 μι Hg/ml 9.4 15 2 µl ug/ml Sole >20 6. 10 μι ug/ml > >Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following directions. Add 8 mL of diluent to yield 10mL of reconstituted solution. How many mL will you give?
- Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at 50ml/he using the 15 drop factor?When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the final concentration of the lysosome solution after adding 8.0 of a biuret reagent to the solution 2.5mg/ml 1mg/ml 0.5mg/ml 5 mg/mlI. How much of a 10 mg/mL lysozyme stock would you need to add to 4 mL of cells for a final concentration of 1 mg/mL? What is the lysozyme doing?
- Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.2.58. Bacteria have equivalent diameters of 2 × 10-6 m and densities of approximately 1 kg/L. Under optimal conditions, bacteria can di- vide every 30 min. Determine the mass of bacteria that would accumulate in 72 hr under continuing optimal growth conditions. Can this occur? Explain.After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.
- 1:55 .ll 0.52 KB/S 56 1.pdf CHEM 3141: BioChemistry Quiz no. 1- Answer key I.) Draw the following compounds. 1.) Maltose: 2 Glucose, a-(1,4) 2.) Gentobiose: 2 Glucose, B-(1,6) 3.) C-2 epimer of Talose 4.) C-3 epimer of Fructose 5.) C-2 epimer of ribose II. Predict the products of the following reactions, if there is none, write NO RXN. Also indicate, if the reaction is fast or slow он FO HO -H H -OH но. + Resorcinol/dilute HCI 1.) 2.) Sucrose + Copper (II) Acetate/Acetic Acid HO H HO H HO H H OH 3.) HO + Orcinol/HCI10-12. For a solution in which u = 8.0 X 102, calculate %3D K for *(a) AgSCN. (b) РЫ2 s "(c) La(IO3)3. lde (d) MgNH,PO4. spWhat's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45