Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: What is the Gram stain test procedure? What important diseases are caused by the following bacteria:…
A: The Gram staining is the the basic staining procedure that is used to identify bacterial. This…
Q: A bacterial organism is used to inoculate mannitol broth medium. Following incubation the broth is…
A: A nutrient growth medium is a type of solution or mixture that is designed to provide nutrients to…
Q: Why is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for…
A: A physiological saline solution known as Hank's balanced salt solution (HBSS) is used to keep cells'…
Q: You are hired in one of the microbiology labs in Memphis and your first assignment to identify…
A: The last reagent used in Gram staining, which is crucial for differentiating between Gram-positive…
Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…
A: Microbiology is the branch of science which deals with the study of different types microorganisms…
Q: What are high level disinfectants? What is an example of one? What is the method of action and the…
A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…
Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…
A: Smear preparation is the first step carried out during the staining procedure.
Q: Write a paragraph explaining the Gram stain result and the differences in the endospores of these…
A: The Gram staining procedure is used to classify bacterial species into two broad groups namely…
Q: Is the pH level of antibacterial soap higher, lower, or the same as H. influenzae, S. aureus and S.…
A: Antibacterial soap These are the soaps which have chemical ingredients that have the ability to…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: Control of Microbial Growth A) Which would be the best process to use and why? B) Can you use…
A: A. In the control of microbial growth, Heating is the best method for controlling microbial growth,…
Q: You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do…
A: Differential media is a type of growth media used in the labs to grow and differentiate or…
Q: 6) The autoclave desired Temperature to kill bacterial spores must be left for at least ____ in…
A: An autoclave is a device used in laboratory and medical settings to sterilize equipment, materials,…
Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…
A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…
Q: What are the characteristics of an ideal antimicrobial agent?
A: Microorganisms have a very large world. Some are beneficial to live creatures, while others are…
Q: why is it important that you disinfect your work area both before and after working with bacteria?
A: Bacteria are present everywhere in each and every corner,present on non-living surfaces and as well…
Q: The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture…
A: Petridish method is the growing or culturing the microorganisms in the petridish. It is easier to…
Disinfectant A is most effective as there is no growth of salmonella choleraesuis at dilution 1:2 ,1:4 or 1:8.
Step by step
Solved in 2 steps
- How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible unknown? Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis. Create flow chart (dichotomues) using at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test.Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?Considering microbial identification methods, to which category would the following method belong based on what it measures? * Performing a hydrogen peroxide breakdown assay to see if a microbe produces the enzyme catalase Serological O Biochemical Genotypic Phenotypic
- Salmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) False
- For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against E.coli and S.aureus.For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. What are the concentrations of antibiotic applied in the test?The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0
- The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1Below is an MSA plate inoculated with bacteria and grown at 37*C for 2 days. Be pecific and thorough in your answers for parts a and b below. The color of the plate is yellow because b) The presence of bacterial colonies indicates that the bacteria can surviveWhen a disinfectant or antiseptic's effectiveness is expressed as a phenol coefficient, a number ____ 1.0 means the chemical agent is more effective than phenol. less than greater than equal to