Glucose used up Lactose used up Glucose + Lactose added Bacterial density (cells/mL) 72 2 VA VB 00 74 5 Time (h) 115 10 VD

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.

 

Glucose
used up
Lactose
used up
Glucose +
Lactose
added
Bacterial density (cells/mL)
72
2
VA
VB
00
74
5
Time (h)
115
10
VD
Transcribed Image Text:Glucose used up Lactose used up Glucose + Lactose added Bacterial density (cells/mL) 72 2 VA VB 00 74 5 Time (h) 115 10 VD
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