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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
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- Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following directions. Add 8 mL of diluent to yield 10mL of reconstituted solution. How many mL will you give?Sucrose initial Final delta %weight change Conc. weight weightweightincreaseX100/origi 1.41 6.41 28.2 0.1 6.38 1.38 27.6 0.2 5.80 0.80 16 0.3 5.05 0.05 1 0.4 4.50 0.5 |10 0.5 4.00 1 20 0.6 3.5 |1.5 30 0.7 2.90 2.1 42 0.8 2.20 2.8 0.9 |1.75 3.25 1.0 1.38 3.62 |72.4 Then 9. Determine the molar concentration of sucrose that gives 0% change in weight. Obtain the Ys using the previous formula. 10. Then compute the water potential using the fomula: Yw = Ys + 4p Your answer will be in units of J m3 (energy per unit volume) which are equivalent to pascal (Pa). Convert to MPa by dividing to 106. What is the sucrose concentration at which zero percent change in weight is observed: What is the water potential Yw of Tradescantia or Allium сера сells? What is the water potential Yw of the Solanum tuberosum potato cells? 6536 55 555555 5
- Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at 50ml/he using the 15 drop factor?Environment Cell Scenario and a glucose concentration of 0.3M. The "environment" has a sucrose concentration of 0.2M and a glucose concentration of 0.7M. The The "cell" has a sucrose concentration of 0.4M membrane is permeable to sucrose and the membrane is permeable to glucose. 1.5 Will the "cell" become lyzed, turgid, normal, flaccid, shrivel or plasmolyzed? State the result for an animal cell and for a plant cell. 1.6 What is the final concentration of sucrose and glucose in the cell and in the environment?Hi again! So sorry. I have submitted a few questions on this. I understand the concepts of lactic acidosis fermentation/industrial fermentation but filling in the blanks always fumbles me up. I just can't seem to make the wording "match" or somewhat fit into the context of the sentence :/ so frustrating. Any help would be so appreciated
- 5 900uL of lysozyme solution was added with 100uL of Fraction X into a cuvette. In a kinetic measurement using spectrophotometer, the Abs/min value for lysozyme cell wall hydrolysis activity was -0.040. The settings were at 450nm, 3 second intervals. Calculate the concentration of lysozyme activity that is present, in units of abs/min/mL. If you could show and explain how to calculate, that would be amazingin food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45
