For the study of alanine production by a recombinant strain of E. coli, cultivation was carried out in a benchtop bioreactor with 4.5 L of culture medium, using glucose as a limiting substrate. During the cultivation, there was no lag phase and the cells showed exponential growth for 5 hours. The following table presents the results of the analysis of ammonia and glucose consumption, and alanine accumulation throughout the cultivation. Knowing that 500 mL of a cell suspension at a concentration of 5.0 g/L (inoculum) was added to the 4.5 L of medium in the reactor and that the YX/NH3 previously determined was 7.5, calculate:a) the maximum specific growth rateb) YX/S and YP/S yield factorsc) How long would it take to reach Cx = 30 g/L if the cells continued with the exponential growth profile until the end of the culture (without nutrient deprivation or any type of inhibition)?d) Describe how the mathematical treatment of the data should be done to determine the type of product formation kinetics time (h) NH3(g/L) glucose alanine 0 5 20 0,2 2 4,92 19 0,52 5 4,57 14,7 1,91 8 3,73 5,8 3,76 10 3,22 0 5,2
For the study of alanine production by a recombinant strain of E. coli, cultivation was carried out in a benchtop bioreactor with 4.5 L of culture medium, using glucose as a limiting substrate. During the cultivation, there was no
a) the maximum specific growth rate
b) YX/S and YP/S yield factors
c) How long would it take to reach Cx = 30 g/L if the cells continued with the exponential growth profile until the end of the culture (without nutrient deprivation or any type of inhibition)?
d) Describe how the mathematical treatment of the data should be done to determine the type of product formation kinetics
| time (h) | NH3(g/L) | glucose | alanine |
| 0 | 5 | 20 | 0,2 |
| 2 | 4,92 | 19 | 0,52 |
| 5 | 4,57 | 14,7 | 1,91 |
| 8 | 3,73 | 5,8 | 3,76 |
| 10 | 3,22 | 0 | 5,2 |
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