Various concentrations of recombinant human insulin were prepared for use standards foran HPLC method. To verify the prepared concentrations, the samples were analyzed bymeasuring the absorbance at 280 nm in a short path length (5 mm) cuvette. The molarabsorption coefficient for human insulin is approximately 5.875 x 10³ M-¹cm-¹.a. Calculate the extinction coefficient in mL mg-¹cm-¹.b. Calculate the concentrations of the following human insulin standards if the measuredabsorbances and dilutions used are:Standard 1Standard 2Abs. (at 280 nm) of Diluted Sample0.3050.685Dilution145.0 µL sample, 25.0 μL buffer130.0 µL sample, 40.0 μL buffer

Beer's Law states that the absorbance is proportional to concentration of the given solute which is…

Answered: Various concentrations of recombinant…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?
You want to anesthetize a rat using Nembutal (a type of barbiturate). The effectivedose is 10 mg/Kg Nembutal, the rat weighs 250 g and the Nembutal solution contains 1mg/ml. How many ml of Nembutal do you inject?
Suppose that you are tasked to determine the protein concentration of an unknown protein solution via Bradford assay. You prepared six solutions of bovine serum albumin (BSA) with different concentrations. The initial concentration of the BSA stock solution is 7.50 mg/mL. Approximately 200 µL of Bradford Reagent was added to each of these solutions and the absorbance at 595 nm was taken after 5 minutes. See the table below for data on the standard solutions. Standard # A595 BSA conc (mg/mL) 0.000 0.158 2 1.125 0.291 2.250 0.372 4 3.375 0.503 5 4.500 6 5.625 0.675 Determine the protein concentration, in mg/mL, of the unknown solution if its absorbance at 595 nm was 0.248. Note: Final answer format must be x.xx (two decimal places). Round off only in the final answer. Do not round off in the middle of calculation.
Consider the following set of data and answer the fo
The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b = RNA (µg) 0.00 10.36 20.72 31.08 41.44 Absorbance at 260 nm 0.268 0.497 0.795 1.131 1.373 Hig Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.699.
An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are: a. Is prostigmine competitive or noncompetitive inhibitor? b. determine the value of km and rmax for the two experiments, compare
You obtained the following raw data when setting up a Bradford standard curve: Absorbancy BSA (mg/ml) 595nm 0.225 1 0.310 2 0.420 0.510 4 0.610 0.720 6 0.810 0.915 8 0.950 0.980 10 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.594 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
A colleague has successfully purified the enzyme sphingomyelinase from bovine brain. She provides you with the following incomplete purification table: Specific Activity (nmol/min) (nmol/min/mg) (%) Purification Factor (-fold) Total Total Purification Step Activity Yield Protein (mg) 100 1 Whole homogenate 40-60% (NH4);SO4 CM-sepharose ConA-sepharose Gel filtration Isoelectric focusing 88,625 5,685 925 544 51,700 32,067 29,500 31,680 1,855 420 5 0.03 (A) (B) (C) Per your colleague's request, you agree to calculate: (A) the final specific activity, (B) the final % yield, and (C) the final fold-purification. - x x x x *xx x x
If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?
A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).
Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absorbs at 340 nm, and Y absorbs at 480 nm. A.) At what wavelength would you measure the change in absorbance to assay for enzyme X? Would the absorbance increase or decrease over time? B.)If Vmax = 60 μmol/min and you used 400 μL of a 0.1 mg/mL solution of enzyme, what is the turnover number?
If the enzyme maltase has a Vo of 0.25 mM per minute when [S] = 0.10 mM, and a Vo of 0.40 mM perminute when [S] = 0.40 mM, what is its y-intercept on a Lineweaver-Burk plot?A. 0.10 minutes per mMB. 0.20 minutes per mMC. 0.50 minutes per mMD. 1.0 minutes per mME. 2.0 minutes per mM

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