1. Consider the following set of data and answer the following questions: IS) (M) 6x 10 1x 105 2x 105 6x 105 1.8 x 10 V (umol/min) V (+ Inhibitor) (umol/min) 20.8 12 29 15 45 20 67.6 24 87 28 a. Plot the data on a Lineweaver-Burk plot (be sure to label axes) b. Determine the Km C. Determine the Vmax d. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and detemine the new Km and Vmax and the type of inhibitor (competitive, uncompetitive, non-competitive). e. Can the effects of the inhibitor be over-ridden by adding more substrate? Why?
1. Consider the following set of data and answer the following questions: IS) (M) 6x 10 1x 105 2x 105 6x 105 1.8 x 10 V (umol/min) V (+ Inhibitor) (umol/min) 20.8 12 29 15 45 20 67.6 24 87 28 a. Plot the data on a Lineweaver-Burk plot (be sure to label axes) b. Determine the Km C. Determine the Vmax d. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and detemine the new Km and Vmax and the type of inhibitor (competitive, uncompetitive, non-competitive). e. Can the effects of the inhibitor be over-ridden by adding more substrate? Why?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Consider the following set of data and answer the fo
![2.
Consider the following set of data and answer the following questions:
() tsl
V (umol/min)
20.8
V (+ Inhibitor) (umol/min)
6x 10
1 x 105
2x 105
12
62.
45
1-
6x 105
1.8x 10
20
24
67.6
87
28
a. Plot the data on a Lineweaver-Burk plot (be sure to label axes)
b. Determine the Km
c. Defermine the Vmax
d. The second set of velocities represents the rate of the reaction when
an inhibitor is added. Plot these data on the same graph as above
and determine the new Km and Vmax and the type of inhibitor
(competitive, uncompetitive, non-competitive).
e. Can the effects of the inhibitor be over-ridden by adding more
substrate? Why?
The kinetics of an enzyme were analyzed in the absence of inhibitors, as
well as in the presence of Inhibitor A and Inhibitor B. Using the given data
below, construct or calculate the following (Make sure to label graphs
with appropriate axes and equations, and circle final answers):
No Inhibitor
Inhibitor A
Inhibitor B
[S] mM
Vo (mmol/min) V, (mmol/min)
2.40
1.29
3.
2.40
3.60
12
8.40
5.10
24
10.8
6.30
12.0
7.20
8.55
a. Write out the Michaelis-Menten and Lineweaver-Burke equations
b. Plot all of the data on one plot as Michaelis-Menten şaturation curves
c. Plot all of the data on one plot in the Lineweaver-Burk format
d. Define Km and Vmax
e. Mathematically determine the Km and Vmax in the absence and In the
presence of inhibitors
f. Determine what type of inhibitors A and B are](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ffdb5e394-61b9-4828-b452-834cf319525d%2F848cf736-7495-408b-9760-645659142742%2Flb2t42s.jpeg&w=3840&q=75)
Transcribed Image Text:2.
Consider the following set of data and answer the following questions:
() tsl
V (umol/min)
20.8
V (+ Inhibitor) (umol/min)
6x 10
1 x 105
2x 105
12
62.
45
1-
6x 105
1.8x 10
20
24
67.6
87
28
a. Plot the data on a Lineweaver-Burk plot (be sure to label axes)
b. Determine the Km
c. Defermine the Vmax
d. The second set of velocities represents the rate of the reaction when
an inhibitor is added. Plot these data on the same graph as above
and determine the new Km and Vmax and the type of inhibitor
(competitive, uncompetitive, non-competitive).
e. Can the effects of the inhibitor be over-ridden by adding more
substrate? Why?
The kinetics of an enzyme were analyzed in the absence of inhibitors, as
well as in the presence of Inhibitor A and Inhibitor B. Using the given data
below, construct or calculate the following (Make sure to label graphs
with appropriate axes and equations, and circle final answers):
No Inhibitor
Inhibitor A
Inhibitor B
[S] mM
Vo (mmol/min) V, (mmol/min)
2.40
1.29
3.
2.40
3.60
12
8.40
5.10
24
10.8
6.30
12.0
7.20
8.55
a. Write out the Michaelis-Menten and Lineweaver-Burke equations
b. Plot all of the data on one plot as Michaelis-Menten şaturation curves
c. Plot all of the data on one plot in the Lineweaver-Burk format
d. Define Km and Vmax
e. Mathematically determine the Km and Vmax in the absence and In the
presence of inhibitors
f. Determine what type of inhibitors A and B are
Expert Solution
Step 1
Hi! Thank you for your questions. As you have asked multiple questions but haven't mentioned which one question is to be answered, we are answering only the first from the top question with its subparts. To get answer for remaining questions, kindly repost them separately.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON