From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearly defined separation between molecules? Explain your conclusions.   Parameters required for calculation of coefficient (Kd) for unknown protein   Volume eluted (mL) Which variable does this volume represent in the equation for Kd? Fraction with maximal DNP-Aspartate detected 36 Vt Fraction with maximal Protein detected 24 Ve Fraction with maximal Blue dextran detected 6 Vo

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
icon
Concept explainers
Question

From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearly
defined separation between molecules? Explain your conclusions.

 

Parameters required for calculation of coefficient (Kd) for unknown protein

 

Volume eluted (mL)

Which variable does this volume represent in the equation for Kd?

Fraction with maximal DNP-Aspartate detected

36

Vt

Fraction with maximal Protein detected

24

Ve

Fraction with maximal Blue dextran detected

6

Vo

Standard curve for known proteins through gel
filtration column using Sephadex G75
4.8
4.6
4.4
4.2
4
y = -1.439x + 4.8152
R2 = 0.9995
3.8
3.6
3.4
3.2
3
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Distribution Coefficient (Kd)
Log10(MW)
Transcribed Image Text:Standard curve for known proteins through gel filtration column using Sephadex G75 4.8 4.6 4.4 4.2 4 y = -1.439x + 4.8152 R2 = 0.9995 3.8 3.6 3.4 3.2 3 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 Distribution Coefficient (Kd) Log10(MW)
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

Blurred answer
Knowledge Booster
Proteins
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON