You repeat your kinetic assay for three additional substrate concentrations and determine the initial velocities using the same approach as above. These values, originally obtained in units of mAbs/s, are then converted into units of nmol/min. A summary of all of measured initial velocities for each amoxicillin concentration is provided in the table. 10 25 50 100 200 300 [Substrate] (μM) 4.8 10.9 18.8 29.5 41.1 47.3 Initial velocity (nmol/min) Draw a graph of initial velocity versus substrate concentration. The labels for the x and y axes are intentionally left blank. Determine the dependent and independent variables before graphing. Label the plot direct.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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8L.Part I

You repeat your kinetic assay for three additional substrate concentrations and determine the initial velocities using the same approach as above. These values,
originally obtained in units of mAbs/s, are then converted into units of nmol/min. A summary of all of measured initial velocities for each amoxicillin concentration
is provided in the table.
10
25
50
100
200
300
+
B
[Substrate]
(µM)
Draw a graph of initial velocity versus substrate concentration. The labels for the x and y axes are intentionally left blank. Determine the dependent and independent
variables before graphing. Label the plot direct.
No elements selected
300
275
250
225
200
175
150
125
100
75
50
25
4.8
10.9
18.8
29.5
41.1
47.3
Y
Initial velocity
(nmol/min)
EDD
?
I
25 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400
Transcribed Image Text:You repeat your kinetic assay for three additional substrate concentrations and determine the initial velocities using the same approach as above. These values, originally obtained in units of mAbs/s, are then converted into units of nmol/min. A summary of all of measured initial velocities for each amoxicillin concentration is provided in the table. 10 25 50 100 200 300 + B [Substrate] (µM) Draw a graph of initial velocity versus substrate concentration. The labels for the x and y axes are intentionally left blank. Determine the dependent and independent variables before graphing. Label the plot direct. No elements selected 300 275 250 225 200 175 150 125 100 75 50 25 4.8 10.9 18.8 29.5 41.1 47.3 Y Initial velocity (nmol/min) EDD ? I 25 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400
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