Which fractions measured from your gel filtration experiment had the most and least amount of protein detected? Which fraction likely contained the most myoglobin based off molecular weight and protein presence? Which fraction likely contained the most egg albumin based off molecular weight and protein presence? Which samples measured from your ion exchange experiment had the most protein detected? Calculate the approximate pI of your proteins using the ion exchange Table 2. Ion Exchange Samples Tested by Bradford Assay Concentration BSA (μg/mL) Concentration BSA (mg/mL) Volume of Sample added to Bradford Reagent (μL) Absorbance at 595 nm Standard 1 250 0.25 50 0.32 Standard 2 125 0.125 50 0.16 Standard 3 62.5 0.0625 50 0.08 Standard 4 31.25 0.03125 50 0.04 Standard 5 15.625 0.015625 50 0.02 Blank 0.00 0.00 0.00 Table 3. Gel Filtration Fractions Tested by Bradford Assay Fraction #: Absorbance at 595 nm Interpolated Concentrations of Protein (μg/mL) 6 0.374 291.96 7 0.395 308.35 8 0.0433 33.80 9 0.170 132.71 10 0.270 210.77 11 0.547 426.01
Structure and Composition of Cell Membrane
Despite differences in structure and function, all living cells in multicellular organisms are surrounded by a cell membrane. Just like the outer layer of the skin separates the body from its environment similarly, the cell membrane, also known as 'plasma membrane,' separates the inner content from its exterior environment.
Cell Membrane
The cell membrane is known by different names like plasma membrane or cytoplasmic membrane, or biological membrane. The term "cell membrane" was first introduced by C. Nageli and C. Cramer in the year 1855. Later on, in 1931, the term "plasmalemma" for cell membrane was given by J. Plowe. The cell membrane separates the cell's internal environment from the extracellular space. This separation allows the protection of cells from their environment.
Prokaryotes vs Eukaryotes
The cell is defined as the basic structural and functional unit of life. The cell membrane bounds it. It is capable of independent existence.
Which fractions measured from your gel filtration experiment had the most and least amount of protein detected?
Which fraction likely contained the most myoglobin based off molecular weight and protein presence?
Which fraction likely contained the most egg albumin based off molecular weight and protein presence?
Which samples measured from your ion exchange experiment had the most protein detected? Calculate the approximate pI of your proteins using the ion exchange
Table 2. Ion Exchange Samples Tested by Bradford Assay
|
|
Concentration BSA (μg/mL) |
Concentration BSA (mg/mL) |
Volume of Sample added to Bradford Reagent (μL) |
Absorbance at 595 nm |
|
Standard 1 |
250 |
0.25 |
50 |
0.32 |
|
Standard 2 |
125 |
0.125 |
50 |
0.16 |
|
Standard 3 |
62.5 |
0.0625 |
50 |
0.08 |
|
Standard 4 |
31.25 |
0.03125 |
50 |
0.04 |
|
Standard 5 |
15.625 |
0.015625 |
50 |
0.02 |
|
Blank |
0.00 |
0.00 |
|
0.00 |
Table 3. Gel Filtration Fractions Tested by Bradford Assay
|
Fraction #: |
Absorbance at 595 nm |
Interpolated Concentrations of Protein (μg/mL) |
|
6 |
0.374 |
291.96 |
|
7 |
0.395 |
308.35 |
|
8 |
0.0433 |
33.80 |
|
9 |
0.170 |
132.71 |
|
10 |
0.270 |
210.77 |
|
11 |
0.547 |
426.01 |
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