Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 20 mM Acetic acid 157.59 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 5% SDS ddH20 95.21 288.37 18.02

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