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Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 20 mM Acetic acid 157.59 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 5% SDS ddH20 95.21 288.37 18.02

Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 20 mM Acetic acid 157.59 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 5% SDS ddH20 95.21 288.37 18.02

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In this experiment, you are to prepare 250mL of Cell Lysis Buffer using the reagents given in Table. Fill out the table. Show your computations at the bottom of the table.

 

Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 157.59 20 mM Acetic acid 1 mM EDTA 60.05 292.24 10 mM MgCl2 from 25mM stock 5% SDS 95.21 288.37 ddH20 18.02
Transcribed Image Text:Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 157.59 20 mM Acetic acid 1 mM EDTA 60.05 292.24 10 mM MgCl2 from 25mM stock 5% SDS 95.21 288.37 ddH20 18.02
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