Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 20 mM Acetic acid 157.59 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 5% SDS ddH20 95.21 288.37 18.02
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In this experiment, you are to prepare 250mL of Cell Lysis Buffer using the reagents given in Table. Fill out the table. Show your computations at the bottom of the table.
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- Rx Of oxacinophtalmic solution 3% Disp. 10 mL How many milligrams of axacin contained in each milliter of the dispensed prescription?Composition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02Penicillin G potassium 5000 units Isotonic Sodium Chloride Solution ad 15 mL Sig. Nose drop Using soluble penicillin tablets, each containing 100,000 units of crystalline penicillin G potassium, explain how you would obtain penicillin G potassium needed in compounding the prescription.
- CAR) All una Red stock soluton concentration: wis strue Somple #yelume dilution 1 ete total tock AR volume 50.00m 50. 00mL 50.00L AR COC conentrastion Absorbance Q dilutibn 2 dilution 3 10 O. 16.4 20 0. 237 turn dilution 4 25 So00 O. 392 shift unknarn 1 0.485 (Include a printed copy of the colibratlon curve) Colibrotion urve cayotion: R2 valvePaclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL X
- A 15kg doe diagnosed with pregnancy toxaemia requires a dextrose fluidsupplementation. Given a dosage of 0.2ml/kg of body weight, prepare a 5%dextrose solution from a stock solution of 50%.After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.Siturul alORK ING )A mother brings her 251b child Into the ER with q very high fevero she has already ad ministered l2 4sp of Tylenol at home. She has brought the bottle with her and the label states: Acetamaino phere syrmp 325 mg / 5mLo Administer 10-15 mg /89 every 4 hrs as needed . How many milligrams oL Tylenot has she givern? Was this asafe dose to give the child?
- 10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein Concentration 15.2 6.6 2 3.75 4.7 (mg/mL) Final Sample Volume 60 30 20 4 3 (mL) Enzyme Specific Activity 0.43 1.7 7.25 18.3 12.5 (units/mgprotein) Based on your protein purification sample data, in which of the purification steps in your protocol did you effectively purify your enzyme? Select all that apply. a) anion exchange correct b) cation exchange correct c) size exclusion 1 correct d) size exclusion 2