Final Protein Concentration: 8 uM Final Gdn HCI Concentration (8 M stock) uL Gdn HCI stock uL HAstV-1 stock uL Storage Buffer Final Volume (uL) 50 0.25 50 0.5 50 1 50 1.5 50 2 2.5 25 50 50 3 3.5 4 4.5 5.25 5.5 5.75 6 50 50 50 50 50 50 50 50 50
Fill the table given the info below. Please thank you.
Protein at 1 mg/mL which is also 1g/L. Use the DNA sequence below to export to ExPASy Translate & ProtParam. (I tried doing so and I calculated a Molarity of 16.51uM which came from the 60,567.28Da from ExPASy). Using the molarity then use it to fill out the table.
atgggcgaggagtataaagttgtcctcacatttggatccccaatgagccctaatgcaaataacaaacagacttgggttaataaacctcttgatgcgccttcgggccattacaatgtgaaaattgcaaaggatgttgaccactatctaaccatgcagggtttcacttctatagcatctgttgactggtacactatagattttcaaccatctgaggcgcctgccccgataaaaggcttgcaggtacttgtgaacatctcgaaaaaagctgatgtgtatgccgtcaaacaatttgtcacagcgcagaccaacaacaagcaccaggttacaagcctgttcctagtaaaagtaacaactggttttcaggtgaacaactacctgagttacttttacagggcgtctgctactggggatgccacaactaacctgttggttagaggagacacatacacagcagggataagttttacccagggtggatggtatttgttgacaaatacatctattgttgatggggctatgccacctggctgggtctggaataacgtggaacttaaaactaacacagcgtatcacatggacaaaggcttggtccatctaataatgcctttgcctgagtccacgcaaatgtgttatgagatgctgacatctattccagcggccgcagagctcgctctggtgccacgcggtagttccgctcatcaccaccatcatcaccatcaccaccact
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