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You and your lab partners decide to redo the B-galactosidase lab. You prepare three test tubes: Test Tube A contains 5.0 mL of Luria Broth (LB) Test Tube B contains 4.5 mL of LB and 0.5 mL of 4% lactose Test Tube C contains 4.5 mL of LB and 0.5 mL of 4% glucose You pipette 200 uL of E. coli bacteria into each test tube, mix them, and incubate them at 37°C for 20 minutes. After this incubation period, you retrieve three microcentrifuge tubes and the provided b-gal blank. You remove 250 uL each from Test Tubes A, B, and C and add it to your labeled microcentrifuge tubes, then add 20 uL of PopCulture and allow them to incubate at room temperature for 8 minutes. When 8 minutes are up, you add 500 uL of Z-buffer and 100 uL of ONPG and let the tubes incubate at room temperature for 10 minutes. Lastly, you add 150 uL of Na2CO3 before measuring the B-galactosidase activity at the spectrophotometer. Which volume would you use in your calculation of units of enzyme activity? 250 uL 150 uL 500 uL 20 uL 200 uL 100 uL

You and your lab partners decide to redo the B-galactosidase lab. You prepare three test tubes: Test Tube A contains 5.0 mL of Luria Broth (LB) Test Tube B contains 4.5 mL of LB and 0.5 mL of 4% lactose Test Tube C contains 4.5 mL of LB and 0.5 mL of 4% glucose You pipette 200 uL of E. coli bacteria into each test tube, mix them, and incubate them at 37°C for 20 minutes. After this incubation period, you retrieve three microcentrifuge tubes and the provided b-gal blank. You remove 250 uL each from Test Tubes A, B, and C and add it to your labeled microcentrifuge tubes, then add 20 uL of PopCulture and allow them to incubate at room temperature for 8 minutes. When 8 minutes are up, you add 500 uL of Z-buffer and 100 uL of ONPG and let the tubes incubate at room temperature for 10 minutes. Lastly, you add 150 uL of Na2CO3 before measuring the B-galactosidase activity at the spectrophotometer. Which volume would you use in your calculation of units of enzyme activity? 250 uL 150 uL 500 uL 20 uL 200 uL 100 uL

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You and your lab partners decide to redo the B-galactosidase lab. You prepare three test tubes: Test Tube A contains 5.0 mL of Luria Broth (LB) Test Tube B contains 4.5 mL of LB and 0.5 mL of 4% lactose Test Tube C contains 4.5 mL of LB and 0.5 mL of 4% glucose You pipette 200 uL of E. coli bacteria into each test tube, mix them, and incubate them at 37°C for 20 minutes. After this incubation period, you retrieve three microcentrifuge tubes and the provided b-gal blank. You remove 250 uL each from Test Tubes A, B, and C and add it to your labeled microcentrifuge tubes, then add 20 uL of PopCulture and allow them to incubate at room temperature for 8 minutes. When 8 minutes are up, you add 500 uL of Z-buffer and 100 uL of ONPG and let the tubes incubate at room temperature for 10 minutes. Lastly, you add 150 uL of Na2CO3 before measuring the B-galactosidase activity at the spectrophotometer. Which volume would you use in your calculation of units of enzyme activity? 250 uL 150 uL 500 uL 20 uL 200 uL 100 uL
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