When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is visible. There are no other proteins visible on the rest of the gel. Did something go wrong here? Why can’t you see the other proteins?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is visible. There are no other proteins visible on the rest of the gel. Did something go wrong here? Why can’t you see the other proteins?
![The image depicts a person holding a transparent gel plate with gloved hands. The gel contains multiple colored bands, which appear to be part of a chromatography experiment.
The text "LADDER" with an arrow is labeled on the image, pointing towards the left side of the gel plate, indicating a molecular weight ladder. This ladder serves as a standard reference to help identify the size of other molecules separated on the gel. The bands are arranged vertically, each representing a different molecule's position within the gel. This setup is typical in electrophoresis, a technique used to separate macromolecules like DNA, RNA, or proteins based on size.
The color and intensity of the bands likely vary, showcasing different concentrations and types of molecules present in each lane of the gel.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff37ed8c2-1239-4436-aee0-35b7cc6728fa%2F386ec01e-ab47-4130-91f6-2362fdc14070%2Fvvb1d1m_processed.png&w=3840&q=75)
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