When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is visible. There are no other proteins visible on the rest of the gel. Did something go wrong here? Why can’t you see the other proteins?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is visible. There are no other proteins visible on the rest of the gel. Did something go wrong here? Why can’t you see the other proteins?

The image depicts a person holding a transparent gel plate with gloved hands. The gel contains multiple colored bands, which appear to be part of a chromatography experiment. 

The text "LADDER" with an arrow is labeled on the image, pointing towards the left side of the gel plate, indicating a molecular weight ladder. This ladder serves as a standard reference to help identify the size of other molecules separated on the gel. The bands are arranged vertically, each representing a different molecule's position within the gel. This setup is typical in electrophoresis, a technique used to separate macromolecules like DNA, RNA, or proteins based on size.

The color and intensity of the bands likely vary, showcasing different concentrations and types of molecules present in each lane of the gel.
Transcribed Image Text:The image depicts a person holding a transparent gel plate with gloved hands. The gel contains multiple colored bands, which appear to be part of a chromatography experiment. The text "LADDER" with an arrow is labeled on the image, pointing towards the left side of the gel plate, indicating a molecular weight ladder. This ladder serves as a standard reference to help identify the size of other molecules separated on the gel. The bands are arranged vertically, each representing a different molecule's position within the gel. This setup is typical in electrophoresis, a technique used to separate macromolecules like DNA, RNA, or proteins based on size. The color and intensity of the bands likely vary, showcasing different concentrations and types of molecules present in each lane of the gel.
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