SDS-PAGE and Agarose gel electrophoresis can both be used to separate proteins or protein- complexes based on their size. Separation of a multi-subunit protein complex by SDS-PAGE resulted in two bands with molecular weights of 86 KDa and 136 KDa, while separation of the same complex using Agarose gel electrophoresis resulted in two bands with molecular weights of 222 KDa and 444 KDa. Based on this information, which of the following statements is most likely to be correct? O The complex has a molecular weight of 222 KDa The complex exists as a dimer of homo-dimers The complex exists as a dimer of hetero-dimers The complex exists as a trimer, but the individual protein subunits are covalently crosslinked to each other

Q: Explain Peptide Mapping and Internal Sequencing of Proteins from Acrylamide Gels.

A: Peptide mapping is an identity test for proteins, those obtained by rDNA technology. It involves the…

Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?

A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino…

Q: Mammalian cells growing in culture were labeled with [3H]-thymidineto estimate the rate of DNA…

A: Mammalian cells growing in culture were labeled with [3H]-thymidineto estimate the rate of DNA…

Q: Answer all whether True or false for the statements given below with a valid reason. a) Ethidium…

A: Apologies. As per norms, we are entitled to do only the first three subparts of a question. I am…

Q: If you have the following proteins? Protein MW, kDa 67 pl 3.5 A В 45 7.3 C D 45 5.0 14.5 7.3 E 67…

A: Proteins B and D have the same PI. However there is a considerable difference in the size of these…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: A chain of biochemical events is responsible for Aequorea victoria turning green. Firstly, the…

A: Gel filtration chromatography, or size exclusion chromatography, is a chromatographic technique for…

Q: A good way to increase total proteome coverage without using 2D PAGE is to:     "Use two,…

A: Proteins are often separated and recognized using a variety of separation techniques such as size…

Q: Distinguish between native gel electrophoresis of protiens and SDS-PAGE of proteins in terms of the…

A: gel electrophoresis is the a biotechnological technique used to separate biological molecules based…

Q: uring incubation, prepare dilutions of the standard antiserum which constitutes the standard curve…

A: To prepare a standard curve for the assay, we need to dilute the standard antiserum to a range of…

Q: When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is…

A: SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), is commonly used as a method…

Q: Consider the equation below. What are its consequences in the context of an electrophoresis…

A: Electrophoresis is a technique used in laboratories to separate DNA, RNA, and protein molecules…

Q: Estimate the time needed for E. coli RNA polymerase at 37 °C to transcribe the entire gene for a…

A: To solve this question,  Average molecular mass of an amino acid is 110 dalton. Average rate of…

Q: List 4 advantages of biological value of protein determination method.

A: Protein structure involves three-dimensional structure and arrangement of the sequence of amino…

Q: You have an aqueous solution containing: Alaine (a mono-amino, monocarboxylic acid) Fructose (a…

A: An assay can be defined as an analytic procedure that is used in laboratory medicine, mining for…

Q: You are trying to determine the cell count per microliter of some melanoma cells that have been…

A: Stock solutions often have a very high concentration of cells which makes it manually impossible to…

Q: You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be…

A: Beers law states that the absorbance of a sample depends on the amount of sample present and path…

Q: Analysis of a 49kDA protein is aimed with a western blot technique. For this purpose, "whole cell…

A: Western blot is a technique in which the concentration of a protein is determined using SDS PAGE…

Q: Describe the common strategy (steps) for protein sequencing, starting with a biological sample…

A: Proteins are one of the 4 major biomacromolecules. Proteins are the most abundant of the 4…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.

A: Gel electrophoresis is a laboratory technique, which is used to separate mixtures of DNA or RNA…

Q: You are trying to isolate a protein you don't know much about, so you decide to use a gel column…

A: Introduction :-  Chromatography is a biophysical technique which is used to separate or isolate the…

Q: A. In replicate analyses, the carbohydrate content of a glycoprotein ( a protein with sugars…

A: According to the given data, the glycoprotein having 11.9g of carbohydrate content in 100g of…

Q: If your analysis identifies that the total Cytosine content in a sample is 29%, what will be the…

A: According to Chargaff's rule number of purine(adenine+guanine) = number of pyrimidine (cytosine +…

Q: Expressing genes encoding a particular protein’s subunits from a plasmid in E. coli cells (volume of…

A: Escherichia coli (E. coli) are group of bacteria that are prevalent in the environment, foods, and…

Q: You're purifying some plasmid DNA from a culture of bacteria and you want to know how pure it is.…

A: The purity of the resulting DNA must be assessed when plasmid DNA is purified from a bacterial…

Q: c) Let us consider the following toehold DNA system: 2 2* 1/2 12 In textual form this is given by…

A: In the provided scenario, question (c) examines the impact of extending the toehold length from 4 to…

Q: Estimate the viscosith of 1.0 vol % agarose gel solution if it took 58 minutes for 1500 base pairs…

A: In order to solve this problem, we need to first calculate electrophoretic mobility () during the…

Q: Estimate the time needed for E. coli RNA polymerase at 37 °C to transcribe the entire gene for a…

A: Introduction: E.coli is a kind of bacteria that typically lives in your intestines. They are mainly…

Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…

A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…

Q: How can gel-filtration chromatography be used to arrive at an estimate of the molecular weight of a…

A: Chromatography is an important analytical technique that contributes to the separation,…

Q: --TGT(G/C)CAG------3'

A: SNV means single nucleotide variants on individual RNA molecules To identify genomic differences we…

Q: A student conducted an experiment on protein isolation from mungbean. After isolating the protein,…

A: Bradford assay is an example for a general protein assay. It is used to quantify the unknown amount…

Q: r DNA isolation using Qiagen extraction kit, the ophotometric measurements. Explain the situation i)…

A: The deoxyribonucleic acid extraction method frees deoxyribonucleic acid from the cell then}…

Q: 1a) In MALDI-TOF data, when performing peptide mass mapping search,  the peptide masses and the…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: You add 3.0 µg of plasmid DNA to a QIAprep spin miniprep purification column. You perform the…

A: Negative charge of silanol: A silica membrane is commonly seen in QIA prep spin mini prep…

Q: You start with 100 units of protein activity and 100 grams of total protein. After the first…

A: Protein purification is performed to purify a target protein from a mixture of proteins. Fold…

Q: Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) is used to identify the…

A: Electrophoretic separation of proteins is most commonly performed in polyacrylamide gels. The…

Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.