If you know how many molecules of DNA you have, and you know the molecular weight of each molecule, you can calculate how many grams of DNA you have using the Avogadro constant (6.02 x 1023). Suppose you're doing a plasmid purification like the one you did for PGLO. You start with 1.5 ml of bacterial culture containing 108 cells per ml. Each cell contains 100 copies of the plasmid, and the plasmid is 5.4 kb. How much plasmid DNA is present in your 1.5 ml culture, in terms of ng? (Show ALL your calculations on a piece of paper, take a picture and paste it in the space below).

If you know how many molecules of DNA you have, and you know the molecular weight of each molecule, you can calculate how many grams of DNA you have using the Avogadro constant (6.02 x 1023). Suppose you're doing a plasmid purification like the one you did for PGLO. You start with 1.5 ml of bacterial culture containing 108 cells per ml. Each cell contains 100 copies of the plasmid, and the plasmid is 5.4 kb. How much plasmid DNA is present in your 1.5 ml culture, in terms of ng? (Show ALL your calculations on a piece of paper, take a picture and paste it in the space below).

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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