A chain of biochemical events is responsible for Aequorea victoria turning green. Firstly, the protein aequorin converts chemical energy into blue light. A second protein, known as GFP (green fluorescent protein), absorbs the light. Aequorin is a 21 kDa protein with a pI of 4.5 that generates blue light from an attached non-protein chromophore. GFP is a 27 kDa protein with a pI of 6.2. In order for it to fluoresce, 3 amino acids undergo a remarkable reaction when they are folded in the right way. Aequorin does not absorb light in the visible range, and GFP absorbs light at 395 nm and 475 nm. 1. Sketch what you would see if you ran a mixture of aequorin and GFP over a size-exclusion column. In your sketch, include absorbance data at 280, 395, and 475 nm.
A chain of biochemical events is responsible for Aequorea victoria turning green. Firstly, the protein aequorin converts chemical energy into blue light. A second protein, known as GFP (green fluorescent protein), absorbs the light. Aequorin is a 21 kDa protein with a pI of 4.5 that generates blue light from an attached non-protein chromophore. GFP is a 27 kDa protein with a pI of 6.2. In order for it to fluoresce, 3 amino acids undergo a remarkable reaction when they are folded in the right way. Aequorin does not absorb light in the visible range, and GFP absorbs light at 395 nm and 475 nm.
1. Sketch what you would see if you ran a mixture of aequorin and GFP over a size-exclusion column. In your sketch, include absorbance data at 280, 395, and 475 nm.
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