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Beer's Law to determine Protein Concentration You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be 1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficient and molecular weight of p53 is 35,410 M-1 cm-1 and 43,653 Da, respectively (l = 1 cm)

Beer's Law to determine Protein Concentration You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be 1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficient and molecular weight of p53 is 35,410 M-1 cm-1 and 43,653 Da, respectively (l = 1 cm)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Beer's Law to determine Protein Concentration

You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be
1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficient
and molecular weight of p53 is 35,410 M-1 cm-1 and 43,653 Da, respectively (l = 1 cm).

Example Problem: Using Beer's Law to Determine Protein Concentration You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be 1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficient and molecular weight of p53 is 35,410 M¹cm¹ and 43,653 Da, respectively (I = 1 cm).
Transcribed Image Text:Example Problem: Using Beer's Law to Determine Protein Concentration You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be 1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficient and molecular weight of p53 is 35,410 M¹cm¹ and 43,653 Da, respectively (I = 1 cm).
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