The last residue of the protein (tail) is Tryptophan, and the first residue (head) is labeled with IAEDANS acceptor. Estimate the length of the protein (head to tail) if the efficiency of RET measures at 0.01
The last residue of the protein (tail) is Tryptophan, and the first residue (head) is labeled with IAEDANS acceptor. Estimate the length of the protein (head to tail) if the efficiency of RET measures at 0.01
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
The last residue of the protein (tail) is Tryptophan, and the first residue (head) is labeled with IAEDANS acceptor. Estimate the length of the protein (head to tail) if
the efficiency of RET measures at 0.01
Please show step by step and how to get the 34.014 number
![**Table 20.2: Values of R₀ for some donor–acceptor pairs**
| Donor | Acceptor | R₀/nm |
|------------|----------|-------|
| Naphthalene | Dansyl | 2.2 |
| Dansyl | ODR | 4.3 |
| Pyrene | Coumarin | 3.9 |
| IAEDANS | FITC | 4.9 |
| Tryptophan | IAEDANS | 2.2 |
| Tryptophan | Haem | 2.9 |
**Abbreviations:**
- **Dansyl:** 5-dimethylamino-1-naphthalenesulfonic
- **FITC:** fluorescein-5-isothiocyanate
- **IAEDANS:** 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid
- **ODR:** octadecyl-rhodamine
The table presents donor–acceptor pairs, showing their respective Förster distance (R₀) values, which indicate the efficiency of energy transfer processes between the pairs. These values are measured in nanometers (nm), and they are crucial in fields such as fluorescence resonance energy transfer (FRET) analysis.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9382baff-1bd2-45e2-9fb5-327d0d7cc8e7%2Fe356ff25-3fe8-4b73-9453-ea8fe76f4d4e%2Fy79rzvo_processed.jpeg&w=3840&q=75)
Transcribed Image Text:**Table 20.2: Values of R₀ for some donor–acceptor pairs**
| Donor | Acceptor | R₀/nm |
|------------|----------|-------|
| Naphthalene | Dansyl | 2.2 |
| Dansyl | ODR | 4.3 |
| Pyrene | Coumarin | 3.9 |
| IAEDANS | FITC | 4.9 |
| Tryptophan | IAEDANS | 2.2 |
| Tryptophan | Haem | 2.9 |
**Abbreviations:**
- **Dansyl:** 5-dimethylamino-1-naphthalenesulfonic
- **FITC:** fluorescein-5-isothiocyanate
- **IAEDANS:** 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid
- **ODR:** octadecyl-rhodamine
The table presents donor–acceptor pairs, showing their respective Förster distance (R₀) values, which indicate the efficiency of energy transfer processes between the pairs. These values are measured in nanometers (nm), and they are crucial in fields such as fluorescence resonance energy transfer (FRET) analysis.
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