Proteinase K should be used as the first step of DNA purification (before applying phenol CIA mixture) to remove most proteins. Proteinase K alone cannot remove all proteins from the lysate. Explain why the Proteinase K enzyme cannot remove ALL proteins from the cell lysate.

Q: Why is polyacrylamide gel is used in electrophoresis?

A: Introduction  Electrophoresis is a widely used laboratory technique for separating and analyzing…

Q: Which of the following plates could represent the results of the transformation experiment with PGLO…

A: INTRODUCTION Transformation is a genetic exchange mechanism by which “naked” DNA is taken up by…

Q: Why is mouse spleen organn pressed and passed  through a cell strainer in splenoyte preparation?

A: Splenocytes are vital for immunology research because they are easily isolated and used in a wide…

Q: Why can’t Agrobacterium be used for transforming all plants?

A: Agrobacterium is a phytopathogen tool used for plant transformation in agriculture. Agrobacterium…

Q: You and your lab partners decide to redo the B-galactosidase lab. You prepare three test tubes:…

A: The correct volume to use in the calculation of units of enzyme activity is 250 uL. This volume…

Q: Choose an enzyme or therapeutic protein of high market value. Explain the biological process used to…

A: Glucocerebrosidase is a lysosomal enzyme that catalyzes the breakdown of glucocerebroside intosugar…

Q: | | || | || a. Label each lane of the gel. Write only the corresponding letters in the wells above.…

A: Introduction Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein…

Q: A phenylgalactoside-type compound, which yields galactose when enzymatically cleaved, was added as…

A: An operon is a functional unit of genomic DNA that comprises a collection of genes that are all…

Q: Which of the protein purification methods listed below is the first step one needs to take after…

A: Introduction: Protein purification involves the separation and purification of proteins from a…

Q: TRUE OR FALSE: Mupirocin inhibits bacterial cell wall synthesis. Mupirocin is used as a negative…

A: we have to answer wether the given statement is true or false . Due to question answering policies…

Q: Bacillus subtilis is known to harbour proA gene which is expressed as protease. Protease is an…

A: Genetic engineering and bioprocess are very good tools in the pharmaceutical, therapeutics, enzyme…

Q: A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments…

A: Electrophoresis is a technique used to separate charged particles like protein, RNA, and DNA. It is…

Q: Elaborate on the abbreviation RTP. What does it stand for? What is it used for?

A: We all know the contribution of proteins in our body.Now it may help in getting energy.Proteins can…

Q: You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of…

A: Viable cell means living cells whereas non-viable cells are dead cells. Viable cells of bacteria…

Q: Restriction enzyme:   Recognition sequence:     Number of pieces of DNA:  Type of cut:

A: In the question a DNA sequence is given with its nucleotides.  Restriction enzyme is an enzyme that…

Q: Figure 5: MCF-7 cells were incubated for 24 h with the drug dilution buffer (control) or various…

A: Microtubules are made up of protein tubulins that can undergo quick growth or dissolution by…

Q: A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to…

A: High titer lysate:            A concentrated liquid sample of phage obtained by the infection of the…

Q: Has 53' DNA polymerase activity [Choose] [Choose ] RNA polymerase DNA polymerase I Coats…

A: Replication is the first and most important stage in the central dogma of the cell machinery. In…

Q: Alkaline phosphatase conjugated to the anti-DIG antibody recognizes the Dig-labeled probe. The…

A: Introduction  Alkaline phosphatase (AP) is an enzyme that can catalyze the hydrolysis of various…

Q: TRUE OF FALSE: Is E coli colonies would be pink mucoid in Eosin Methylene Blue Agar (EMBA)? Is…

A: EMBA or Eosin Y methylene Blue Agar is used for the identification of Gram-negative bacterial…

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: In this talk, we will learn about a very important experiment called the Avery, McLeod, and McCarty…

Q: Mild detergents, like Triton X-100, would be the best for isolation of peripheral proteins from the…

A: The cell membrane is a structure which protects the cell and acts as a barrier and transports the…

Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…

A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…

Q: You got an opportunity to join a professor lab who is working in-vivo model and specifically looking…

A: Mitochondria are called the power house of the cells as it is the site where the maximum amount of…

Q: The production of purple pigment in a certain species of flower is dependent upon two gene loci. You…

A: A dominant trait is expressed in both homozygous and heterozygous genotypes.

Q: You will be setting up your diagnostic digest on three plasmids, the ones you began with, PGFPUV and…

A: In this question, we have to complete the table for the reaction mixture of a PCR experiment.

Q: What protein purification methods can be used to ensure that the resulting protein is soluble ?

A: The method used for protein purification that can be used to ensure that the resulting protein is…

Q: Your colleague handed you a novel strain of coli that is purifying a protein with a 6xHisTag; they…

A: Detailed ExplainationCompetitive Metal Chelation:Dicarboxylic acids, such as citric acid, have…

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.