When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reason for such an observation?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reason for such an observation?
Zymography is a technique to assess the enzymatic activity of proteins either in situ or by separating them with electrophoresis. The enzyme converts the substrate into a product which is detected by different staining methods. Example: Polyacrylamide gel electrophoresis.
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