Since higher concentration colcemid will result in shorter chromosome, you want to      change your protocol and  reduce final concentration of colcemid in your 10 ml blood      culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with      concentration10ug/ml needed to be added in 10ml blood culture?

Q: To determine whether proteins interact with each other which two techniques might you use 1. Yeast…

A: Protein-protein interactions (PPIs) are highly-specific physical contacts that occur between two or…

Q: Why is the plate count method a determination of the number of viable cells?

A: The plate count method, also referred to as the viable count method or colony-forming unit (CFU)…

Q: Which of the following parameters should be altered to increase the amount of vector-insert…

A: In molecular biology research, ligation is a fundamental technique used to join DNA fragments…

Q: In lab we learned a technique that helped us to visulize individual colonies of bacter 1. Describe…

A: Introduction :- Bacterial colonies are visible clusters of bacteria that arise from a single…

Q: How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell…

A: Surface layer (S- layer) is the self assembled monomeric proteins forming to protect the surface of…

Q: You have conducted serial 10-fold dilutions and measured the cfu (colony forming units) of a…

A: Given information CFU for the bacterial sample= 5 colonies volume of the sample= 0.4 ml Dilution=…

Q: Graph SEQ Graph ARABICI p24 po/mi 2) Quantitative skills: Using the graph below, determine the…

A: In the question, the graph is given in which different molecular are shown. It is asked to determine…

Q: You will be given the plaque assay plates to analyse from three different bacteriophages stocks You…

A: It's crucial to select the best phage for the job while carrying out mutagenesis research utilising…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Biochemical pathway will be as-  MM- MM+C - MM+A - MM+B - CM Hint- the more the positive signs of…

Q: Differentiate the following artificial media used in animal cell culture: serum-containing media…

A:

Q: On the four compounds testes for antibacterial activity using agar well diffusion assay, a. Which…

A: Antibacterial is an activity performed by any chemical or other compound through which it will the…

Q: You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a…

A: A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to…

Q: You perform a plaque assay with your bacteria and serial dilutions of your bacteriophage,, and then…

A: Serial dilution is a process of making a series of dilution in order to determine the concentration…

Q: You have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted 35…

A: Given  Volume plated = 0.1ml  Total dilution = 108  Number of colony = 35

Q: In a COVID test, why is it necessary to swab all the way at the back of the nose and throat

A: The COVID-19 pandemic has currently affected the whole world and has claimed a huge number of lives.…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much…

A: Serial dilution is one of the fundamental techniques carried out in chemical and biology…

Q: Is the isolation plate pigmented? (is their a color for it)? what is colony morphology(single cells…

A: Colony morphology is a method that is used to describe the characteristics of an individual colony…

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: If the concentration of phage in the original sample was markedly increased from the concentration…

A: 1. Dilution Series:  - Start with a high dilution, such as 10^-5 or 10^-6, of the original phage…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase…

A: Enzymes can be defined as proteins that act as biological catalysts. They work by accelerating…

Q: A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL…

A: It is given that a culture of Staphylococcus is diluted as follows:1. The original 20mL of culture…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Auxotrophic microorganisms cannot produce a certain organic chemical essential for their growth. The…

Q: 1) both streak plating and pour plating produce isoluated colonies. What is the underlying…

A: Hello there! Since you have posted multiple questions and not mentioned which one of them to be…

Q: 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that can replicate…

Q: This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the…

A: Ans: Recombinant DNA technology: The technique of cutting and pasting of DNA molecule with another…

Q: The equilibration step is adding the serum sample to the buffer-equilibrated (pH 6) magnetic Protein…

A: Immunoglobulins are glycoproteins that is purified using protein G. Protein G has specific site for…

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: You want to create a 10-fold dilution . You have a saline tube with a volume of 450ul. How much of…

A: According to this theory, a diluted solution's volume will be 10 times greater than its stock…

Q: What would happen if an ampicillin reagent is added to an Lysogeny broth agar plate that is below 50…

A: Ampicillin is an antibiotic which enables the synthesis of the peptidoglycan of bacterial cells wall…

Q: 1 trpt cells trp- cells + DNase 3 2 trp cells DNA 4 TSA plate 1 trpt cells trp- cells + DNA 3 2 trp…

A: In scientific experiments, a control is an experimental group or sample that is used as a comparison…

Q: What are the steps in sandwich ELISA? Explain and provide a schemiatic diagram

A: Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as…

Q: Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is…

A: Joshua Lederberg created the term "plasmid" in 1952. Plasmids are  genetic elements which are…

Q: cm^2 adn when confluent contains 2 x 10^7 cells). What is the generatoin time (doubling time)? A.…

A:

Q: Which method of counting cells would you use for each of these situations? Viable Cell Count Optical…

A: In microbiology counting bacterial cells is an important technique for several research and…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to…

A: Introduction: Phagehunter is the study of unidentified bacteriophages using bioinformatics,…

Q: Why would you use one single colony for both streaks on a plate and inoculate a culture for plasmid…

A: Introduction Plasmids are important for the transformation of bacteria. They are used as vectors and…

Q: What is the purpose of making a serial dilution?

A: Given: In microbiology, serial dilutions (log dilutions) are used to reduce the bacterial…

Q: a) Make a drawing of the cells from each culture to identify the various stages of the cell…

A: Saccharomyces cerevisiae is a eukaryotic organism that is used in various scientific experiments. It…

Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: n the Methyl Red-Voges Proskauer test, the samples were not transferred into sterile tubes for…

A: These tests are done to check which fermentation pathway is taken by pathogen to ferment glucose.…

Q: Colony of (bacteria): A cluster of cells (or clones) which arise from a single bacterium by asexual…

A: