This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be done by the following technique. O 1) Plate on nutrient agar plates that contain ampicillin. 2) Plate on nutrient agar plates that contain both ampicillin and tetracycline. O 3) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin. 4) Plate on nutrient agar plates that contain tetracycline. 5) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline.

Q: Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal…

A: Restriction endonucleases also referred to as restriction enzymes are enzymes that cut DNA at…

Q: Which would you choose if you needed to make a partial diploid for complementation analysis? rolling…

A: The correct answer is option 1. Rolling circle plasmid.

Q: The principle of the alkaline method for plasmid DNA isolation is: O A. An acidic solution…

A: Plasmid DNA isolation by alkaline lysis method This method is the most commonly used method to…

Q: Provide 2 diagrams to illustrate the 2 potential recombinant plasmids,

A: The question here states about the concerned recombination of cloned plasmids and their…

Q: The compact ball of capillaries in a nephron is called:- a. the nephron loop. b. the peritubular…

A: Nephron contains a typical ball-like structure that has numerous capillaries. It is studied under…

Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…

A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…

Q: Comment on the failure of p1vir transduction experiments and give a reason why you think the…

A: transduction experiments is a technique commonly used in molecular biology to introduce foreign DNA…

Q: A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent)…

A: The transformation is the natural process in which the donor bacteria releases the free DNA in the…

Q: Single and double digestion of plasmid pJC716 were performed using the restriction enzymes EcoRI and…

A: Plasmid is a double stranded circular dna molecule that is capable of replicating independently. It…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

Q: Looking at the model data i upload and given the question how could i analyse tboth experimental…

A: Agarose gel electrophoresis is the separation of DNA on the basis of size, supercoiled DNA will move…

Q: Ms. Carla was required to clone three different genes (Gene 1, Gene 2 and Gene 3) into PUC18. After…

A: Blue-white screening technique that can be used to identify the recombinant bacteria which have…

Q: explain the method of Identifying mutant genes by plasmid librarytransformation

A: Transformation is the horizontal gene transfer by which some bacteria can take up foreign genetic…

Q: Conjugation depends on direct cell-to-cell contactbetween a donor F+ carrying either a…

A: Conjugation is the process of genetic material transfer between two bacterial cells through direct…

Q: The transfection reagent used to introduce plasmids to the HEK293 cells was a lipid reagent. Why is…

A: Transfection is the incorporation of DNA, RNA, or proteins in to the eukaryotic cells for the…

Q: Why is it possible to visualize a PCR product on an agarose gel even if the template genome is…

A: Gel electrophoresis is a technique used in the separation of macromolecules such as proteins and DNA…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: The ____ plasmid contains genes for synthesizing connections between donor and recipient cells.

A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…

Q: In this western blot, the levels of phosphorylated TBK (PTBK) decrease with increasing…

A: Western blot analysis is the assay done to check expression of protein. In this method, proteins are…

Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?

A: The technology used for joining different DNA molecules from different species is known as…

Q: A plasmid provides its host with kanamycin resistance. What will happen to the plasmid if you…

A: Plasmids are defined as extrachromosomal circular genetic elements that are capable of…

Q: 1 3. 4 Two different cultures of Escherichia coli bacteria were inoculated onto nutrient agar…

A: pGLO plasmid comprises the gene for Green Fluorescent Protein (GFP) and a gene for resistance to the…

Q: Which plasmid contains the therapeutic gene? 1 - the plasmids with SERCA2a 2- the viral plasmid 3-…

A: The adenoviruses are generally used for making recombinant animal cells.

Q: What do you mean by vector plasmid?

A: Bacteria can be defined as the single-celled microscopic organism which is cosmopolitan as it is…

Q: Why might it be advantageous for a bacterial cell to receive the F plasmid from another cell?

A: The F plasmid, the fertility factor is a large plasmid that consists of genes that allow the…

Q: Jm109 cells transformed with ligation and plasmid alone is used. The colonies most like:   white…

A: β-galactosidase is an enzyme encoded by the lacZ gene and is used to break down lactose to glucose…

Q: Please answer this question in short but concise and cohesive way. "How would you select the host…

A: Recombinant DNA technology involves the introduction of a particular gene of interest into a host…

Q: Agraobacterium mediated transformation is a useful gene transfer technique but still we can’t use…

A: Agrobacterium species harboring tumor-inducing (Ti) or hairy root-inducing (Ri) plasmids cause crown…

Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…

A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…

Q: Distinguish between a plate with individual colonies and a plate with a lawn. Under what conditions…

A: Introduction  A pure culture is a population of cells or multicellular organisms growing in the…

Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

A: The blue–white screen is a techinque that allows for the rapid and convenient detection of…

Q: The properties that all plasmids share are that they1. all carry genes for antimicrobial…

A: Plasmids are extrachromosomal nucleic acids that are found generally outside the host's genetic…

Q: What is the role of the following in the alkaline plasmid screen?    G buffer (Cell Suspension…

A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…

Q: Explain how you would use the blue-white colony screening technique to identify bacteria containing…

A: Plasmids are one of the most commonly used vector in recombinant DNA technology.This technology…

Q: Why is it necessary to prepare positive and negative control reactions in restriction digestion of a…

A: In restriction digestion of a plasmid (circular DNA molecule), preparing positive and negative…

Q: The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific…

A: Lambda phage exhibits a lytic or lysogenic cycle depending on the expression of certain genes.

Q: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)

A: Plasmids are the extrachromosomal material that exist apart from the main chromosome in a bacterial…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.