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You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have ident the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (parti map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme HindIII and clone the digested DNA in your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7q31.1 H Wells ori FOXP2 1500bp 2kb 1kb 200bp Hindi MCS Size Colony Colony Colony Colony Colony Colony Standard LaiZ PUC-123 H amp --300kb - H-Hind!!! H XRAY H 600bp 900bp Insert Check-Gel of your samples H

You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have ident the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (parti map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme HindIII and clone the digested DNA in your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7q31.1 H Wells ori FOXP2 1500bp 2kb 1kb 200bp Hindi MCS Size Colony Colony Colony Colony Colony Colony Standard LaiZ PUC-123 H amp --300kb - H-Hind!!! H XRAY H 600bp 900bp Insert Check-Gel of your samples H

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. How large is your plasmid

2. Which colony(-ies) is(are) likely to have been white?

3. Which colony(-ies) is (are) likely to have the XRAY gene? 

 

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You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hind/II and clone the digested DNA into your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7q31.1 H FOXP2 H H XRAY H H + --300kb -- H-Hind!!! 1500bp 600bp Size Colony Colony Colony Colony Colony Standard 1 2 3 4 2kb 1kb 200bp Insert Check-Gel of your samples MCS Latz PUC-123 Wells ori amp 900bp Colony 6
Transcribed Image Text:You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hind/II and clone the digested DNA into your plasmid (pUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7q31.1 H FOXP2 H H XRAY H H + --300kb -- H-Hind!!! 1500bp 600bp Size Colony Colony Colony Colony Colony Standard 1 2 3 4 2kb 1kb 200bp Insert Check-Gel of your samples MCS Latz PUC-123 Wells ori amp 900bp Colony 6
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