Hind III'Hind III.9 kb.9 kbBam HIplasmid A.9 kb.7kbBam HI.3 kbplasmid B.9 kb.4 kb.3 kb.5 kb/Eco RIBgl Il.4 kbEco RI.7 kb.5 kb-Bgl IIBam HIEco RIYou requested that a lab run by a friend ofyours share a couple of plasmids that theycreated with you. She was willing to sendyou the ones that you asked for inexchange for citation in any papers thatwere published using the plasmids.Eco RIHowever, the labels from two vials havefallen off in the shipping because theadhesive used to hold them was not ableto withstand the temperature, beingshipped on dry ice.Each vial contained a different plasmid andnow you do not know which plasmid is inwhich. Fortunately, you have restrictionmaps for both plasmids, shown above.Explain how you would determine whichvial contained each plasmid and give agraphical representation of yourexpectations related to your method ofBam HI discovery. (25pts)

Answered: Explain how you would determine which…

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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Hind III'
Hind III
.9 kb
.9 kb
Bam HI
plasmid A
.9 kb
.7kb
Bam HI
.3 kb
plasmid B
.9 kb
.4 kb
.3 kb
.5 kb/
Eco RI
Bgl Il
.4 kb
Eco RI
.7 kb
.5 kb
-Bgl II
Bam HI
Eco RI
You requested that a lab run by a friend of
yours share a couple of plasmids that they
created with you. She was willing to send
you the ones that you asked for in
exchange for citation in any papers that
were published using the plasmids.
Eco RI
However, the labels from two vials have
fallen off in the shipping because the
adhesive used to hold them was not able
to withstand the temperature, being
shipped on dry ice.
Each vial contained a different plasmid and
now you do not know which plasmid is in
which. Fortunately, you have restriction
maps for both plasmids, shown above.
Explain how you would determine which
vial contained each plasmid and give a
graphical representation of your
expectations related to your method of
Bam HI discovery. (25pts)

Expert Solution

Step 1

Since both the plasmids are of equal size (3.7kb) we have to double digest both the plasmids with restriction enzymes having single cut site. In both the plasmids we have single restriction sites for Hind III and Bgl II. So, these two enzymes should be used for restriction digestion.

Upon double digestion, we will have the following digestion products.

Plasmid A: 2kb + 1.7kb fragments

Plasmid B: 1.2kb + 2.5 kb fragments.

Running the digestion products on agarose gel and identifying the fragments we can identify the plasmid.

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