Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A plasmid extraction is a process in which DNA is extracted and purified. A plasmid DNA is short extracellular DNA found in some organisms. Mostly they are circular in shape and contain gene for some proteins that help organisms to survive.
If we use water instead of 70% ethanol during the extraction process we loose DNA. Water dissolve the DNA molecules and we loose the isolated DNA. And more, ethanol is use to remove excess salt because ethanol act as solvent for salts but not for DNA while water act as solvent for both DNA as well as salts.
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