Which of the following scenarios would ONLY occur if your skipped the digest purification step? UV/VIS spectrophotometric quantification of DNA may be skewed by uncut plasmid. Some plasmid molecules may be cut once, by one enzyme, and re-ligate to themselves. The fragments cleaved by the restriction enzymes on the plasmid and insert can re-ligate to their sites, causing reduced ligation efficiency. Plasmid molecules cut with EcoRI and Xbal can ligate to each other instead of the insert.

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: Restriction digestion and Gel electrophoresis: A single strand of a double-stranded DNA sequence is…

A: Restriction digestion is a process in which DNA is cut into smaller pieces at specific sites with…

Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…

A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…

Q: PGLO plasmid DNA solution with the UV lamp. Note yo observations. Using a micropipetto withdraw 10…

A: pGLO plasmid has GFP as reporter gene. This plasmid is used as vector to carry desired gene of…

Q: 2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A…

A: Focus only on EcoR1.   Than focus on EcoR1 and HindIII.   Than focus on EcoR1, HindIII and Pst1

Q: Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences,…

A: The restriction enzymes are the endonuclease enzymes, which cuts the DNA at specific sites called…

Q: The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the…

A: Introduction Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…

Q: Ehat  primer sets could be amplify the following DNA sequence?   AATACGTCGCATGGggatccttttttatgcatg

A: Amplification of DNA sequence is done by the polymerase chain reaction. With the help of DNA…

Q: An optimum ligation reaction should contain approximately 50 ng of vector. Given your concentrations…

A: It is given that an optimum ligation reaction should contain approximately 50 ng of the vector. We…

Q: Restriction enzymes look for palindromic sequences of DNA to cut, how does it recognize those…

A: A restriction enzyme or restriction endonuclease is an enzyme that cleaves/cuts DNA into fragments…

Q: You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an…

A: Gel electrophoresis is the separation of DNA or proteins according to molecular weight. It uses…