2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A circular plasmid molecule (12,000 total base pairs in size) was cut with a series of restriction enzymes and the digestions were size fractionated by agarose gel electrophoresis. Some digestions involved just one enzyme (single digest), some combinations of two enzymes (double digests), and one utilized all three enzymes (triple digest). Agarose gel electrophoresis of the digestions produced bands of the following sizes. Enzyme EcoRI Hindi!! Bands of the Agarose Gel (size in base pairs) 2300 and 9700 4000 and 8000

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2. Restriction Enzyme Mapping - use any resources to assist you including
the hints below.
A circular plasmid molecule (12,000 total base pairs in size) was cut with a
series of restriction enzymes and the digestions were size fractionated by
agarose gel electrophoresis. Some digestions involved just one enzyme (single
digest), some combinations of two enzymes (double digests), and one utilized all
three enzymes (triple digest).
Agarose gel electrophoresis of the digestions produced bands of the following
sizes.
Enzyme
EcoRI
Hindi!!
Pstl
EcoRI and Hindill
EcoRI and Psti
Hindill and Pstl
EcoRI, Hindill, and Pst I
Bands of the Agarose Gel (size in base pairs)
2300 and 9700
4000 and 8000
12,000
800, 1500, 2500, and 7200
2300, 3200, and 6500
4000
800, 1500, 2500, 3200, and 4000
I
Draw a plasmid map showing the location of the restriction enzyme sites
relative to each other for each map. Include all 7 maps in your answer.
Transcribed Image Text:2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A circular plasmid molecule (12,000 total base pairs in size) was cut with a series of restriction enzymes and the digestions were size fractionated by agarose gel electrophoresis. Some digestions involved just one enzyme (single digest), some combinations of two enzymes (double digests), and one utilized all three enzymes (triple digest). Agarose gel electrophoresis of the digestions produced bands of the following sizes. Enzyme EcoRI Hindi!! Pstl EcoRI and Hindill EcoRI and Psti Hindill and Pstl EcoRI, Hindill, and Pst I Bands of the Agarose Gel (size in base pairs) 2300 and 9700 4000 and 8000 12,000 800, 1500, 2500, and 7200 2300, 3200, and 6500 4000 800, 1500, 2500, 3200, and 4000 I Draw a plasmid map showing the location of the restriction enzyme sites relative to each other for each map. Include all 7 maps in your answer.
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