human populations for part of the β-interferon gene that can easily be scored using PCR amplification followed by a restriction digest with XhoI: · Allele a1 has a 700-bp PCR product with no XhoI restriction sites. · Allele a2 also has a 700-bp PCR product, but due to a single-nucleotide polymorphism it has an XhoI restriction site 300 bp from one of the priming sites. · Allele a3 has a shorter 600-bp PCR product due to a deletion, and lacks an XhoI restriction site. You load the digested PCR products for three children (1, 2, and 3)
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You have identified 3 different alleles in human populations for part of the β-interferon gene that can easily be scored using PCR amplification followed by a restriction digest with XhoI:
· Allele a1 has a 700-bp PCR product with no XhoI restriction sites.
· Allele a2 also has a 700-bp PCR product, but due to a single-
· Allele a3 has a shorter 600-bp PCR product due to a deletion, and lacks an XhoI restriction site.
You load the digested PCR products for three children (1, 2, and 3) along with their parents (M and F), in wells of an agarose gel (rectangles at the top of the diagram below), run the gel, and obtain the following results. The first lane contains a size marker with 100 bp increments; the bottom band is 100 bp long.
For each individual, type in their diploid genotype, showing their two alleles using some combination of a1, a2, and/or a3, separating the two alleles with a comma. (For example, a homozygote for a2 would be entered as a2,a2.)
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